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681105 Vitronectin, Human Plasma

Vitronectin, Human Plasma: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Serum Spreading Factor, S-Protein

681105
  
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      Übersicht

      Replacement Information
      Description
      OverviewNative vitronectin purified from human plasma. Extracellular matrix protein that promotes cell adhesion. Exists as a mixture of 75 kDa and 65 kDa polypeptides. Biochemically and immunologically distinct from fibronectin. Binds to cells through the RGD sequence in its cell-binding domain.
      Intact protein and SDS-PAGE bands react with monoclonal antibodies to vitronectin, bind to heparin, and promote cell adhesion.
      M.W. 65,000 and 75,000.
      Catalogue Number681105
      Brand Family Calbiochem®
      SynonymsSerum Spreading Factor, S-Protein
      References
      ReferencesHayman, E.G., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4003.
      Product Information
      CAS number83380-82-9
      FormLyophilized
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      SourcePrepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      681105 0

      Documentation

      Vitronectin, Human Plasma SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Vitronectin, Human Plasma Analysenzertifikate

      TitelChargennummer
      681105

      Literatur

      Übersicht
      Hayman, E.G., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4003.

      Literaturstellen

      Titel
    • Laura Belvisi, et al. (2005) Biological and Molecular Properties of a New αvβ3/αvβ5 Integrin Antagonist. Molecular Cancer Therapeutics 4, 1670-1680.
      		•
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-May-2008 RFH
      SynonymsSerum Spreading Factor, S-Protein
      DescriptionNative vitronectin purified from human plasma. Extracellular matrix protein that promotes cell adhesion. Exists as a mixture of ~75 kDa and ~65 kDa polypeptides. Biochemically and immunologically distinct from fibronectin. Intact protein and SDS-PAGE bands react with monoclonal antibodies to vitronectin, bind to heparin, and promote cell adhesion.
      FormLyophilized
      Recommended reaction conditions
      Coating Tissue Culture Dishes with Vitronectin Although the optimal concentration for cell attachment and culture may differ for various cell types and for individual cell culture systems, typically concentrations as low as 50 ng/cm2 promote cell attachment and growth. Coating Procedure 1. Dissolve the desired amount of vitronectin in serum-free medium or PBS. The volume should be sufficient to coat the desired surface evenly (e.g. 1 ml for a 35 mm dish, 3 ml for a 60 mm dish). 2. Add the appropriate amount of distilled material to the culture surface. 3. Incubate at room temperature for 1-2 h. 4. Aspirate the remaining media. 5. Rinse the plates carefully. Avoid scratching the bottom surface of the plates. Store in the refrigerator (4°C) damp or air dried under sterile conditions. This protocol is meant to serve only as a guideline and may need to be modified for specific applications.
      SourcePrepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number83380-82-9
      Purity≥95% by SDS-PAGE
      SolubilityDistilled H₂O or aqueous buffers. Equilibrate vial to room temperature prior to reconstitution. Swirl gently to dissolve.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 10026
      ReferencesHayman, E.G., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4003.
      Citation
    • Laura Belvisi, et al. (2005) Biological and Molecular Properties of a New αvβ3/αvβ5 Integrin Antagonist. Molecular Cancer Therapeutics 4, 1670-1680.
      		•