Millipore Sigma Vibrant Logo

58656 Thermolysin, Geobacillus stearothermophilus

View Products on Sigmaaldrich.com
58656
  
Preis wird abgerufen...
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
Bitte erfragen
Eingeschränkte Verfügbarkeit
Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

       

      Kontakt zum Kundenservice

      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Native thermolysin from Geobacillus stearothermophilus. Zinc metalloendoproteinase with specificity toward isoleucine, leucine, methionine and valine. Inhibitors include EDTA, 1,10-phenanthroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Note: 1 KU = 1000 units.

      Note: 1 KU = 1000 units.
      Catalogue Number58656
      Brand Family Calbiochem®
      References
      ReferencesPfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
      Matsubara, H. 1970. Methods Enzymol. 19, 642.
      Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.
      Product Information
      CAS number37246-64-3
      Activity≥7,000,000 PU/g dry weight

      PU = protease unit
      Unit of DefinitionOne protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
      EC number3.4.24.2
      FormLyophilized
      FormulationLyophilized solid containing calcium acetate, sodium acetate, 3X crystallized.
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXH7500000
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      58656 0

      Documentation

      Thermolysin, Geobacillus stearothermophilus SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Thermolysin, Geobacillus stearothermophilus Analysenzertifikate

      TitelChargennummer
      58656

      Literatur

      Übersicht
      Pfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
      Matsubara, H. 1970. Methods Enzymol. 19, 642.
      Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-June-2015 JSW
      DescriptionNative thermolysin from Geobacillus stearothermophilus. A highly thermostable zinc metallo-endoproteinase that exhibits specificity toward isoleucine, leucine, methionine, and valine. Inhibitors include EDTA, 1,10-phenathroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Has an optimal pH in the range 7.0-9.0.
      FormLyophilized
      FormulationLyophilized solid containing calcium acetate, sodium acetate, 3X crystallized.
      Concentration Label Please refer to vial label for lot-specific concentration
      CAS number37246-64-3
      RTECSXH7500000
      EC number3.4.24.2
      Activity≥7,000,000 PU/g dry weight

      PU = protease unit
      Unit definitionOne protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
      SolubilityReconstitute in 42% glycerol adjusted to pH 8.0 with 0.01 N NaOH, containing 0.005% Triton® X-100 detergent.
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Harmful
      Merck USA index14, 9286
      ReferencesPfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
      Matsubara, H. 1970. Methods Enzymol. 19, 642.
      Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.

      Verwandte Produkte & Anwendungen

      Related Products

      Bestellnummer Beschreibung  
      164598 Aminopeptidase M, Porcine Kidney Preis & Verfügbarkeit
      217372 Carboxypeptidase Y, Excision Grade, Yeast Preis & Verfügbarkeit
      324682 Elastase, Porcine Pancreas, High Purity, Crystallized Preis & Verfügbarkeit
      324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi Preis & Verfügbarkeit
      324713 Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus Preis & Verfügbarkeit
      324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Preis & Verfügbarkeit
      5125 Papain, Carica papaya Preis & Verfügbarkeit
      516360 Pepsin, Porcine Stomach Mucosa Preis & Verfügbarkeit
      650211 Trypsin, Excision Grade, Bovine Pancreas Preis & Verfügbarkeit

      Kategorien

      Life Science Research > Proteins and Enzymes > Other Enzymes