71540 MilliporeTag•off™ rEK Cleavage/Capture Kit

This product has been discontinued.

Tag•off™ rEK Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins, followed by rapid affinity-based capture and removal of enterokinase. Following cleavage of the target protein, greater than 99% of Tag•off rEK can be quickly removed from the reaction by affinity capture on EKapture Agarose available in the Tag•off rEK Cleavage Capture Kit. Just add EKapture Agarose to the rEK digest, incubate at room temperature for 5 minutes and remove the resin by spin filtration. Because the same buffer conditions are used for both cleavage and capture, no buffer change is necessary. The Tag•off rEK Cleavage Capture Kit includes the EKapture Agarose and Spin Filters in addition to the same components available with the Tag•off High Activity rEK (enzyme, dilution buffer, cleavage buffer and control protein).