QIA40 Sigma-AldrichTIMP-2 ELISA Kit

Figure 1: Standard Curve

Table 1: Typical Assay Data

Table 2: Intra-Assay Precision

This reflects the precision within an assay (mean values as ng/ml).

Table 3: Inter-Assay Precision

This reflects the precision between assays (mean values as ng/ml).

Table 4: Recovery

The average % recovery of TIMP-2 protein, spiked to levels throughout the range of the assay in various sample types diluted down to 1:4 (plasma, serum, and tissue samples) are shown above.

Table 5: Expected Values

Levels in normal human plasma and serum samples evaluated in the assay.

Table 6: Linearity

The assay linearity was assessed using one serum and one cell culture media sample spiked with known concentrations of TIMP-2 protein in various matrices and diluted with Assay/Wash Buffer. The samples generated values, which fell within the dynamic range of the assay. The linearity assay results are shown abve.

Table 7: Specificity

Table 8: Specificity

The assay can detect TIMP-2 in other species including mouse, rat, guinea pig, rabbit, and bovine. The immunoreactivity between human and rat purified TIMP-2 is equivalent.

Figure 2: Protocol Summary