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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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03-0202-00
MilliporeSMC® Human NF-L High Sensitivity Immunoassay Kit
The SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain Protein (NF-L) in Human Serum, Plasma and Cerebrospinal Fluid (CSF) samples.
More>>The SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain Protein (NF-L) in Human Serum, Plasma and Cerebrospinal Fluid (CSF) samples. Less<<
The SMC® Human NF-L High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Background Information
The SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain Protein (NF-L) in Human Serum, Plasma and Cerebrospinal Fluid (CSF) samples. A capture antibody specific for Human NF-L has been pre-coated onto paramagnetic microparticles (beads). The user pipettes beads, standards, and samples into uncoated microplate wells. During incubation, the NF-L present in the sample binds to the capture antibody on the coated beads. Unbound molecules are washed away during the subsequent wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to NF-L that has been captured onto the beads, thus completing the immunosandwich. Elution buffer is then added and incubated. The elution buffer dissociates the bound protein sandwich from the beads surface releasing the labeled antibodies. These antibodies are separated during transfer to a 384-well read plate. The plate is loaded into the SMCxPRO® System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of NF-L present in the sample when captured. The amount of NF-L in unknown samples is interpolated from a standard curve.
References
Product Information
Components
Assay Buffer
NF-L Coated Beads
Standard Diluent
NF-L Detection Antibody
NF-L Standard
10X Wash Buffer
Buffer D
Elution Buffer B
HS Code
3002 15 90
Precision, %
The assay variations of SMC® Human NF-L Immunoassay kits were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
The SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain Protein (NF-L) in Human Serum, Plasma and Cerebrospinal Fluid (CSF) samples.
Key Applications
Single Molecule Counting (SMC®)
Application Notes
Neurofilament Light Chain (NF-L) is a low molecular weight member of a family of proteins involved in axonal development and function. NF-L accumulation is viewed as a telltale sign of axonal damage in the brain, and is a key marker in a wide range of neurodegenerative diseases, especially as a blood-based biomarker. We are proud to aid these efforts by offering the new SMC® Human NF-L High Sensitivity Immunoassay Kit. By coupling this assay with the powerful state-of-the-art SMCxPRO® detection system, researchers can accurately assess NF-L concentrations even in low abundance in human serum, plasma, and CSF samples, enabling studies that examine the relevance of this key analyte from health to disease.
This new streamline kit has the following features:
• Improved options for sample prep and incubation • Lyophilization protein standard for uniform shipping and storage condition • Increased reagent volumes to accommodate automation • Rigorously developed to achieve accurate and reproducible performance on the state-of-the-art SMCxPRO® ultrasensitive immunoassay system
Biological Information
Cross Reactivity
Cross-reactivity to the following analytes were tested with the following results: NF-M: Not detected NF-H: Not detected pTau (pT181): Not detected Total Tau: Not detected GFAP: Not detected
Accuracy
Minimum of 4 serum samples spiked with average recovery of 93% with a range of 77-105%
Minimum of 4 plasma samples spiked with average recovery of 94% with a range of 78-104%
Minimum of 5 CSF samples spiked with average recovery of 123% with a range of 99-165%
Physicochemical Information
Sensitivity
Lower Limit of Quantification: 0.87 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
