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03-0176-00 SMC™ Human IL-10 High Sensitivity Immunoassay Kit

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03-0176-00
96 well assay  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey Applications
      NhP, M, R, Ca, FeSingle Molecule Counting (SMC®)
      Description
      Catalogue Number03-0176-00
      Trade Name
      • SMC®
      DescriptionSMC™ Human IL-10 High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human IL-10 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin 10 (IL-10) is an anti-inflammatory cytokine derived from T cells, mononuclear phagocytes and keratinocytes with effects on immunoregulation and inflammation. It has shown to inhibit the synthesis of pro-inflammatory cytokines while also suppressing the antigen-presentation capacity of antigen presenting cells. As well, IL-10 stimulates T cells and mast cells as well as B cell maturation and antibody production.
      References
      Product Information
      Components
      • Assay Buffer
      • IL-10 Coated Beads
      • Standard Diluent
      • IL-10 Detection Antibody
      • IL-10 Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • Intra-assay CV% average (n=20): <15%
      • Inter-assay CV% average (n=5): <20%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.
      Key Applications
      • Single Molecule Counting (SMC®)
      Biological Information
      Species Reactivity
      • Non-human Primate
      • Mouse
      • Rat
      • Canine
      • Feline
      Species Reactivity NoteAlthough IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.
      Accuracy
      • Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
      • Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%
      Physicochemical Information
      Sensitivity
      • LLOQ range: 0.1-0.2 pg/mL, refer to CoA for lot-specific LLOQ
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      03-0176-00 04054839753381