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524743 SIRT1, GST-Fusion, Human, Recombinant, E. Coli

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Silent mating type information regulation 2 homolog, Sirtuin1, GST-Fusion, Human, Recombinant, E. coli

524743
  
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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human SIRT1 histone deacetylase consisting of amino acids 193-741 fused at the N-terminus to GST and expressed in E. coli. SIRT1 preferentially deacetylates histones H4 at K16 and H3 at K9 and interacts with and deacetylates histone H1 at K26. More than a dozen non-histone proteins have been found to serve as substrates for SIRT1. It plays a role in several biological processes including stress resistance, metabolism, differentiation, and aging. The active deacetylase is useful in the study of SIRT1 regulation and for inhibitor screening.
      Catalogue Number524743
      Brand Family Calbiochem®
      SynonymsSilent mating type information regulation 2 homolog, Sirtuin1, GST-Fusion, Human, Recombinant, E. coli
      References
      ReferencesKolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873.
      Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enyme required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 30°C.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 20% glycerol, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥80% by SDS-PAGE
      Specific Activity≥8 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      524743 0

      Documentation

      SIRT1, GST-Fusion, Human, Recombinant, E. Coli Analysenzertifikate

      TitelChargennummer
      524743

      Literatur

      Übersicht
      Kolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873.
      Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28.

      Datenblatt

      Titel
      SIRTainty™ Class III HDAC Assay
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-January-2009 JSW
      SynonymsSilent mating type information regulation 2 homolog, Sirtuin1, GST-Fusion, Human, Recombinant, E. coli
      DescriptionRecombinant, human SIRT1 histone deacetylase consisting of amino acids 193-741 fused at the N-terminus to GST and expressed in E. coli. SIRT1 preferentially deacetylates histones H4 at K16 and H3 at K9 and interacts with and deacetylates histone H1 at K26. More than a dozen non-histone proteins have been found to serve as substrates for SIRT1. It plays a role in several biological processes including stress resistance, metabolism, differentiation, and aging. The active deacetylase is useful in the study of SIRT1 regulation and for inhibitor screening.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 20% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 200 µM NAD+, 30 µM fluorescent substrate [Arg-His-Lys-Lys(Ac)], 100 µg/ml BSA, 20 ng/µl SIRT1; incubate at 30°C for 30 min.
      Purity≥80% by SDS-PAGE
      Specific activity≥8 units/µg protein
      Unit definitionOne unit is defined as the amount of enyme required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873.
      Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28.