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12-439 Retinoblastoma Protein (aa 773-928)

Recombinant fusion protein containing the C-terminal fragment (residues 773-928) of human retinoblastoma protein (Rb) with an N-terminal His6-tag, expressed in E. coli.

Retinoblastoma Protein (aa 773-928): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Key Applications: Kinase Assay UniProt Number: P06400 Entrez Gene Number: NM_000321.2
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12-439
500 µg  
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      Übersicht

      Replacement Information
      Description
      Catalogue Number12-439
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRetinoblastoma Protein (aa 773-928)
      OverviewRecombinant fusion protein containing the C-terminal fragment (residues 773-928) of human retinoblastoma protein (Rb) with an N-terminal His6-tag, expressed in E. coli.
      References
      Product Information
      HS Code3502 90 90
      Presentation50 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 500 mM imidazole
      Quality LevelMQ100
      Applications
      ApplicationRecombinant fusion protein containing the C-terminal fragment (residues 773-928) of human retinoblastoma protein (Rb) with an N-terminal His6-tag, expressed in E. coli.
      Key Applications
      • Kinase Assay
      Biological Information
      Concentration2.0 mg/mL
      Entrez Gene Number
      Entrez Gene SummaryRetinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases. The retinoblastoma gene RB was the first tumor suppressor gene cloned, and is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F (MIM 189971) and repress transcription of genes required for S phase (Hanahan and Weinberg, 2000 [PubMed 10647931]).[supplied by OMIM]
      Gene Symbol
      • RB1
      • RB
      • PP110
      • P105-RB
      • retinoblastoma-1
      • OSRC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06400 # Key regulator of entry into cell division that acts as a tumor suppressor. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Also acts as a transcription repressor of E2F target genes by recruiting chromatin-modifying enzymes to promoters. Inhibits the intrinsic kinase activity of TAF1. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding.
      SIZE: 928 amino acids; 106159 Da
      SUBUNIT: Interacts preferentially with transcription factor E2F1. The unphosphorylated form interacts with ARID3B, JARID1A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with KNTC2. Interacts with EID1 and ZUBR1. Interacts with ARID4A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in the retina.
      PTM: Phosphorylated from S to M phase of the cell cycle and is dephosphorylated in G1. T, but not E1A, binds only to the unphosphorylated form.
      DISEASE: SwissProt: P06400 # Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil (cat eye) is investigated. & Defects in RB1 are a cause of bladder cancer [MIM:109800]. & Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].
      SIMILARITY: SwissProt: P06400 ## Belongs to the retinoblastoma protein (RB) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated as a sustrate for many Serine/Threonine kinases.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      12-439 04053252627842

      Documentation

      Retinoblastoma Protein (aa 773-928) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Retinoblastoma Protein (aa 773-928) Analysenzertifikate

      TitelChargennummer
      Rb (amino acids 773-928) - 2318851 2318851
      Rb (amino acids 773-928) - 2497154 2497154
      Rb (amino acids 773-928) - 3704562 3704562
      Rb (amino acids 773-928) - DAM1801488 DAM1801488
      Rb (amino acids 773-928) -2556055 2556055
      Rb (amino acids 773-928) -2635159 2635159
      Retinoblastoma Protein (a.a. 773-928) - 3138333 3138333
      Retinoblastoma Protein (a.a. 773-928) - 3803609 3803609
      Retinoblastoma Protein (a.a. 773-928) - 3803609A 3803609A
      Retinoblastoma Protein (a.a. 773-928) - 3859720 3859720
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