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HTS002RTA Ready-to-Assay™ CXCR2 Chemokine Receptor Frozen Cells

Human CXCR2 / IL-8b GPCR frozen cells for Calcium Flux FLIPR Assays.

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Host Cell: Chem-1 Entrez Gene Number: M73969
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HTS002RTA
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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1CXCR2 / IL-8b
      Description
      Catalogue NumberHTS002RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ CXCR2 Chemokine Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      CXCR2 is a 7-TM G-protein coupled receptor that binds to the chemokines GRO, GRO, GRO, IL-8, ENA-78, NAP-2 and GCP-2 (Olson and Ley, 2002). Neutrophils, mast cells and microvascular endothelial cells express CXCR2, and ligand interactions with CXCR2 promote chemotaxis of these cell types (Heidemann et al., 2003; Nilsson et al., 1999; White et al., 1998). Studies with mice lacking CXCR2 indicate that CXCR2 promotes growth of primary tumors and secondary metastases (Keane et al., 2004), and plays an essential role in hyperoxia-induced lung injury (Sue et al., 2004). In addition, cytomegalovirus encodes a CXCR2-binding chemokine, vCXC-1, that promotes neutrophil migration to infected cells (Penfold et al., 1999). Millipore’s cloned human CXCR2 ]-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CXCR2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CXCR2.
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationHuman CXCR2 / IL-8b GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetCXCR2 / IL-8b
      Target Sub-FamilyChemokine
      Cell Line Type
      • GPCR Cell Lines
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size1 kit
      Global Trade Item Number
      Bestellnummer GTIN
      HTS002RTA 04053252481383