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539791 ProteoExtract® Subcellular Proteome Extraction Kit, Mini

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: S-PEK Kit, Mini

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      Übersicht

      Description
      Overview

      This product has been discontinued.



      The ProteoExtract® Subcellular Proteome Extraction Kit, Mini is a tool for the differential extraction of proteins from mammalian cells according to their subcellular localization. The sequential extraction steps yield proteins in their native state, making it highly suited for demanding proteomics applications. Sample size: 3-5x106 or 25-50 mg tissue.
      Catalogue Number539791
      Brand Family Calbiochem®
      SynonymsS-PEK Kit, Mini
      Materials Required but Not Delivered• Platform mixer (e.g. IKA Vibramax; for extracting adherent tissue culture cells)
      • Cell culture equipment, medium, and other reagents required for cell culture
      • Pipettors and tips for 10 µl, 200 µl, and 1 ml
      • Cooled centrifuge and rotor for 50 ml tube size
      • Cooled microcentrifuge and rotor up to 10,000 x g for 2 ml tube size
      • Thermo mixer or rolling facility
      References
      ReferencesYuan, X., et al. 2002. Electrophoresis 23, 1185.
      Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;
      Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.
      Allen, L., et al. 2000. Nature 405, 819.
      Rabilloud, T. 2000. Anal. Chem. 72, 48A.
      Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.
      Reymond, et al. 1997. Electrophoresis 18, 2842.
      Laemmli, U. K. 1970. Nature 227, 680.
      Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.
      Product Information
      Form10 Extractions
      Kit containsWash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
      Biological Information
      Sample TypeMammalian tissues and cultured cells
      Product Usage Statements
      Intended useThe Calbiochem® ProteoExtract® Subcellular Proteome Extraction Kit Mini (S-PEK) is intended for the differential extraction of proteins from mammalian cells according to their subcellular localization. The procedure yields proteins in their native state making it highly suited for demanding proteomics applications including enzyme activity assays (e.g. reporter gene assays) and subcellular redistribution assays to monitor protein shuttling (e.g. signaling proteins).
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the components of the kit under the following conditions:

      • Wash Buffer: Store at 4°C.
      • Extraction Buffers I-III: Store at 4°C for up to 6 months. For long-term storage, freeze the buffers in convenient aliquots at -20°C.
      • Extraction Buffer IV: Store at room temperature.
      • Protease Inhibitor Cocktail: The cocktail is supplied in DMSO and can be stored at 4°C for up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C.
      • Benzonase® Nuclease: Store at 4°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsWash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
      Global Trade Item Number
      Bestellnummer GTIN
      539791 0