Millipore Sigma Vibrant Logo

539791 ProteoExtract® Subcellular Proteome Extraction Kit, Mini

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: S-PEK Kit, Mini

539791
  
Preis wird abgerufen...
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
—
Bitte erfragen
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

       

      Kontakt zum Kundenservice

      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      The ProteoExtract® Subcellular Proteome Extraction Kit, Mini is a tool for the differential extraction of proteins from mammalian cells according to their subcellular localization. The sequential extraction steps yield proteins in their native state, making it highly suited for demanding proteomics applications. Sample size: 3-5x106 or 25-50 mg tissue.
      Catalogue Number539791
      Brand Family Calbiochem®
      SynonymsS-PEK Kit, Mini
      Materials Required but Not Delivered• Platform mixer (e.g. IKA Vibramax; for extracting adherent tissue culture cells)
      • Cell culture equipment, medium, and other reagents required for cell culture
      • Pipettors and tips for 10 µl, 200 µl, and 1 ml
      • Cooled centrifuge and rotor for 50 ml tube size
      • Cooled microcentrifuge and rotor up to 10,000 x g for 2 ml tube size
      • Thermo mixer or rolling facility
      References
      ReferencesYuan, X., et al. 2002. Electrophoresis 23, 1185.
      Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;
      Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.
      Allen, L., et al. 2000. Nature 405, 819.
      Rabilloud, T. 2000. Anal. Chem. 72, 48A.
      Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.
      Reymond, et al. 1997. Electrophoresis 18, 2842.
      Laemmli, U. K. 1970. Nature 227, 680.
      Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.
      Product Information
      Form10 Extractions
      Kit containsWash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
      Applications
      Biological Information
      Sample TypeMammalian tissues and cultured cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended useThe Calbiochem® ProteoExtract® Subcellular Proteome Extraction Kit Mini (S-PEK) is intended for the differential extraction of proteins from mammalian cells according to their subcellular localization. The procedure yields proteins in their native state making it highly suited for demanding proteomics applications including enzyme activity assays (e.g. reporter gene assays) and subcellular redistribution assays to monitor protein shuttling (e.g. signaling proteins).
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the components of the kit under the following conditions:

      • Wash Buffer: Store at 4°C.
      • Extraction Buffers I-III: Store at 4°C for up to 6 months. For long-term storage, freeze the buffers in convenient aliquots at -20°C.
      • Extraction Buffer IV: Store at room temperature.
      • Protease Inhibitor Cocktail: The cocktail is supplied in DMSO and can be stored at 4°C for up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C.
      • Benzonase® Nuclease: Store at 4°C.
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsWash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      539791 0

      Documentation

      ProteoExtract® Subcellular Proteome Extraction Kit, Mini SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      ProteoExtract® Subcellular Proteome Extraction Kit, Mini Analysenzertifikate

      TitelChargennummer
      539791

      Literatur

      Übersicht
      Yuan, X., et al. 2002. Electrophoresis 23, 1185.
      Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;
      Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.
      Allen, L., et al. 2000. Nature 405, 819.
      Rabilloud, T. 2000. Anal. Chem. 72, 48A.
      Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.
      Reymond, et al. 1997. Electrophoresis 18, 2842.
      Laemmli, U. K. 1970. Nature 227, 680.
      Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.