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539207 Protein G-Agarose

Protein G-Agarose: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
539207
  
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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Protein G binds to the Fc portion of immnuoglobulins (Igs) from various species; useful for binding to Igs that do not react well with protein A. Can be used for antibody immunoprecipitation procedure and to purify immunoglobulins and IgG fractions. Binding capacity: approximately 17-27 mg human IgG/ml gel. Contains approximately 2.5 mg recombinant protein G/ml gel.
      Catalogue Number539207
      Brand Family Calbiochem®
      References
      ReferencesKelley, C.A., et al. 1992. J. Biol. Chem. 267, 2127.
      Sano, T., et al. 1992. Science 258, 120.
      Schwartz, A.L. and Ciechanover, A. 1999. Annu. Rev. Med. 50, 57.
      Voges, D., et al. 1999. Annu. Rev. Biochem. 68, 1015.
      Product Information
      FormLiquid slurry
      Formulation50% suspension in PBS, 20% ethanol, pH 7.0.
      Preservative0.05% sodium azide
      Applications
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      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      539207 0

      Documentation

      Protein G-Agarose Analysenzertifikate

      TitelChargennummer
      539207

      Literatur

      Übersicht
      Kelley, C.A., et al. 1992. J. Biol. Chem. 267, 2127.
      Sano, T., et al. 1992. Science 258, 120.
      Schwartz, A.L. and Ciechanover, A. 1999. Annu. Rev. Med. 50, 57.
      Voges, D., et al. 1999. Annu. Rev. Biochem. 68, 1015.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2008 RFH
      ApplicationImmunoblotting (1:1000)
      DescriptionProtein G binds to the Fc portion of immnuoglobulins (Igs) from various species; useful for binding to Igs that do not react well with protein A. Can be used for antibody immunoprecipitation procedure and to purify immunoglobulins and IgG fractions. Binding capacity: approximately 17-27 mg human IgG/ml gel. Contains ~2.5 mg recombinant protein G/ml gel. The particle size ranges from 45-165 µm with an average size of ~90 µm.
      FormLiquid slurry
      Formulation50% suspension in PBS, 20% ethanol, pH 7.0.
      Recommended reaction conditions
      Purification of IgG from Serum or Ascites using Protein G Agarose Preparation of Solutions: • Loading buffer: 10 mM sodium phosphate, pH 7.0, 150 mM NaCl, 0.05% NaN3 • Elution buffer: 500 mM acetic acid (adjust the pH to 3.0 with 0.005% NH4OH) • Stripping buffer: 1 M acetic acid, pH 2.4 Column Preparation: Pack the column with protein G-agarose (3 ml of agarose per 1 ml of serum or ascites is generally sufficient). Wash with 5 volumes of loading buffer or until the pH of the eluate is 7.0. Sample Loading: Dilute the sample (serum or ascites) with 2 volumes of loading buffer or alternatively dialyze the sample against the loading buffer. Load the sample on the column at a flow rate of 0.5 ml/min. Wash with loading buffer until the absorbance of the eluate at 280 nm approaches background levels. Elution: Elute the IgG by adding the elution buffer. Immediately neutralize the pH of the eluted IgG. Regeneration and Storage: Wash the column with 5 volumes of stripping buffer. Wash with loading buffer until the pH of the eluate is 7.0. Seal the column outlet and store in the refrigerator (4°C). Note: This protocol is meant to serve as a guideline and may need to be modified for specific applications.
      Preservative0.05% sodium azide
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesKelley, C.A., et al. 1992. J. Biol. Chem. 267, 2127.
      Sano, T., et al. 1992. Science 258, 120.
      Schwartz, A.L. and Ciechanover, A. 1999. Annu. Rev. Med. 50, 57.
      Voges, D., et al. 1999. Annu. Rev. Biochem. 68, 1015.