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71113 PopCulture® GST•Mag™ Purification Kit

PopCulture extraction and GST•Tag™ fusion protein purification from E. coli cultures

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      The PopCulture® GST•Mag™ Purification Kit is designed for purification of GST•Tag™ fusion proteins directly from E. coli cultures without harvesting cells. The procedure combines PopCulture total culture extraction with magnetic affinity purification using GST•Mag Agarose Beads.

      PopCulture Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for centrifugation. Use of GST•Mag Agarose Beads enables the entire procedure to be carried out in a single tube without using columns or centrifugation.

      The PopCulture GST•Mag Purification Kit combines PopCulture Reagent, GST•Mag Agarose Beads, corresponding buffers and rLysozyme™ Solution. This kit enables processing of 40 × 3-ml cultures with yields up to 150 µg GST•Tag fusion protein per 3-ml culture, based on bead binding capacity. The kit is compatible with the Novagen Magnetight™ Separation Stand. For 96-well processing using PopCulture, see our various RoboPop™ Purification Kits.

      Description


      Catalogue Number71113 Brand Family Novagen®
      Features and benefits
      • Binding capacity of up to 2 mg/ml
      • Glutathione-derivatized magnetic agarose form with 3 µm average bead size
      • No need to separate cells from culture media: use 25 µl settled beads per milliliter culture (50 µl 50% v/v suspension)
      • No need to mechanically disrupt cells
      • No need to clarify cell extracts prior to purification
      • Direct affinity adsorption of target proteins to resin from the total culture extract
      • Ability to rapidly perform the entire cell growth and purification process in a single tube or well
      References
      Product Information
      •15 mlPopCulture Reagent
      •3 × 1 mlGST•Mag Agarose Beads
      •2 × 100 ml10X GST Bind/Wash Buffer
      •40 ml10X Glutathione Reconstitution Buffer
      •1 gGlutathione, Reduced
      •300 KUrLysozyme Solution
      •1 mlrLysozyme Dilution Buffer
      DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      71113 0