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540020 Pluripotin

Pluripotin: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: N-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII, Ras GTPase-Activating Protein Inhibitor II, SC1

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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      A cell-permeable dihydropyrimido-pyrimidine compound that effectively upregulates GTP-bound active Ras level and inhibits LIF- (leukemia inhibitory factor) induced Erk1/2 phosphorylation in OG2-mES (murine Embryonic Stem) cells (1 µM) via direct interaction with Erk and RasGAP (KD = 212 and 98 nM in binding RasGAP 729-1042 catalytic domain and Erk1, respectively), without affecting cellular STAT3 or GSK-3β/Wnt signaling. Pluripotin/SC1 is shown to greatly complement and enhance the efficiency of LIF in deriving ESCs from blastocysts of several murine strains and effectively maintain the pluripotent state of the ESCs even after 11 passages in cultures. SC1-expanded ESCs effectively form EBs (embryonic bodies) in vitro upon SC1 and LIF withdrawal and are capable of generating chimeric offsprings from surrogate mice when injected into blastocysts from different strains in vivo.
      Catalogue Number540020
      Brand Family Calbiochem®
      SynonymsN-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII, Ras GTPase-Activating Protein Inhibitor II, SC1
      References
      ReferencesYang, W., et al. 2009. Stem Cells 27, 383.
      Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 17266.
      Product Information
      FormYellow solid
      Hill FormulaC₂₇H₂₅F₃N₈O₂
      Chemical formulaC₂₇H₂₅F₃N₈O₂
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥97% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      540020 0

      Documentation

      Pluripotin SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Pluripotin Analysenzertifikate

      TitelChargennummer
      540020

      Literatur

      Übersicht
      Yang, W., et al. 2009. Stem Cells 27, 383.
      Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 17266.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10=February-2011 RFH
      SynonymsN-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII, Ras GTPase-Activating Protein Inhibitor II, SC1
      DescriptionA cell-permeable dihydropyrimido-pyrimidine compound that effectively upregulates GTP-bound active Ras level and inhibits LIF- (leukemia inhibitory factor) induced Erk1/2 phosphorylation in OG2-mES (murine Embryonic Stem) cells (1 µM) via direct interaction with Erk and RasGAP (KD = 212 and 98 nM in binding RasGAP 729-1042 catalytic domain and Erk1, respectively), without affecting cellular STAT3 or GSK-3β/Wnt signaling. Pluripotin/SC1 is shown to greatly complement and enhance the efficiency of LIF in deriving ESCs from blastocysts of several murine strains and effectively maintain the pluripotent state of the ESCs even after 11 passages in cultures. SC1-expanded ESCs effectively form EBs (embryonic bodies) in vitro upon SC1 and LIF withdrawal and are capable of generating chimeric offsprings from surrogate mice when injected into blastocysts from different strains in vivo.
      FormYellow solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₇H₂₅F₃N₈O₂
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesYang, W., et al. 2009. Stem Cells 27, 383.
      Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA 103, 17266.