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525188 Phospholipase C-γ-1, His•Tag®, Human, Recombinant, S. frugiperda

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Phospholipase C-γ-1, Human, Recombinant

525188
  
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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human phospholipase C γ-1 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. PLC-γ-1 is a major substrate for heparin-binding growth factor 1-activated tyrosine kinase. Receptor-mediated activation of PLC-γ-1 and PLC-γ-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. The full-length protein contains the following domains: C2 domain (aa 1075-1177); EF-hand calcium-binding domain (aa 165-176); two PH domains (aa 27-142, aa 4889-523 and aa 895-931); PI-PLC X-box catalytic domain (aa 320-464); PI-PLC Y-box catalytic domain (aa 953-1070); two SH2 domains (aa 550-657, aa 668-756); and a SH3 domain (aa 791-851). Phosphorylation is known to occur at Tyr771 and Tyr1253 by EGFR, Tyr783 by Syk, Ser1248 by PKC-α, and Tyr432 and Tyr775 probably by FGFR.
      Catalogue Number525188
      Brand Family Calbiochem®
      SynonymsPhospholipase C-γ-1, Human, Recombinant
      Application Data
      References
      ReferencesHinsby, A.M., et al. 2004. J. Biol. Chem. 279, 46438.
      Rush, J., et al. 2004. Nat. Biotechnol. 23, 46438.
      Law, C.L., et al. 1996. Mol. Cell. Biol. 16, 1305.
      Lennon, G., et al. 1996. Genomics 33, 151.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      525188 0

      Documentation

      Phospholipase C-γ-1, His•Tag®, Human, Recombinant, S. frugiperda Analysenzertifikate

      TitelChargennummer
      525188

      Literatur

      Übersicht
      Hinsby, A.M., et al. 2004. J. Biol. Chem. 279, 46438.
      Rush, J., et al. 2004. Nat. Biotechnol. 23, 46438.
      Law, C.L., et al. 1996. Mol. Cell. Biol. 16, 1305.
      Lennon, G., et al. 1996. Genomics 33, 151.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-August-2008 RFH
      SynonymsPhospholipase C-γ-1, Human, Recombinant
      Application Data
      DescriptionFull-length, recombinant, human phospholipase C γ-1 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. PLC-γ-1 is a major substrate for heparin-binding growth factor 1-activated tyrosine kinase. Receptor-mediated activation of PLC-γg-1 and PLC-γ-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. The full-length protein contains the following domains: C2 domain (aa 1075-1177); EF-hand calcium-binding domain (aa 165-176); two PH domains (aa 27-142, aa 4889-523 and aa 895-931); PI-PLC X-box catalytic domain (aa 320-464); PI-PLC Y-box catalytic domain (aa 953-1070); two SH2 domains (aa 550-657, aa 668-756); and a SH3 domain (aa 791-851). Phosphorylation is known to occur at Tyr771 and Tyr1253 by EGFR, Tyr783 by Syk, Ser1248 by PKC-α, and Tyr432 and Tyr775 probably by FGFR.
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Irritant
      ReferencesHinsby, A.M., et al. 2004. J. Biol. Chem. 279, 46438.
      Rush, J., et al. 2004. Nat. Biotechnol. 23, 46438.
      Law, C.L., et al. 1996. Mol. Cell. Biol. 16, 1305.
      Lennon, G., et al. 1996. Genomics 33, 151.