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569372 PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6)

This PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr⁴¹⁸).

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src.
      Catalogue Number569372
      Brand Family Calbiochem®
      Application Data
      Detection of mouse Src, phosphorylated on Tyr423, by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells cultured under serum-free conditions, left untreated (lanes 1), or treated with H2O2 (lane 2). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml) Detection: chemiluminescence.

      Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (left lane) or treated with vanadate (right lane). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml). Detection: chemiluminescence.

      Detection of human Src, phosphorylated on Tyr418, by immunocytochemistry. Sample: A431 cells treated with EGF for 15 min. Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (1 µg/ml). Detection: fluorescence.
      References
      ReferencesMartin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
      Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
      Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
      Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
      Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.
      Product Information
      FormLyophilized
      FormulationLyophilized from 2X PBS, PEG, sucrose.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunocytochemistry (1 µg/ml, immunofluorescence)
      Application CommentsDoes not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src
      ImmunogenHuman
      Clone9A6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      569372 0

      Documentation

      PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) Analysenzertifikate

      TitelChargennummer
      569372

      Literatur

      Übersicht
      Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
      Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
      Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
      Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
      Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunocytochemistry (1 µg/ml, immunofluorescence)
      Application Data
      Detection of mouse Src, phosphorylated on Tyr423, by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells cultured under serum-free conditions, left untreated (lanes 1), or treated with H2O2 (lane 2). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml) Detection: chemiluminescence.

      Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (left lane) or treated with vanadate (right lane). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml). Detection: chemiluminescence.

      Detection of human Src, phosphorylated on Tyr418, by immunocytochemistry. Sample: A431 cells treated with EGF for 15 min. Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (1 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 (human, rat), Tyr423 (mouse), and Tyr416 (chicken, v-Src).
      BackgroundSrc belongs to a family of 9 tyrosine kinases which regulate cellular responses to extracellular stimuli. Src is a non-receptor protein tyrosine kinase that transduces signals that are involved in the control of a variety of cellular processes such as proliferation, differentiation, motility, and adhesion. Src is normally maintained in an inactive state, but can be activated transiently during cellular events such as mitosis, or constitutively by abnormal events such as mutation (i.e. v-Src and some human cancers). Src becomes activated upon dephosphorylation of Tyr527 which leads to a conformational change of the Src molecule. This allows binding to other proteins via the SH2 and SH3 domain. Activated Src phosphorylates a variety of substrates involved in the mitogenic signaling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication and RNA processing.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src
      Clone9A6
      IsotypeIgG₁
      Specieshuman, mouse, rat
      FormLyophilized
      FormulationLyophilized from 2X PBS, PEG, sucrose.
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesMartin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
      Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
      Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
      Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
      Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies