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525286 PhosphoDetect™ Anti-Phosphothreonine Mouse mAb (14B3)

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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes phosphothreonine-containing proteins. Supplied with a positive control.
      Catalogue Number525286
      Brand Family Calbiochem®
      Application Data
      Detection of phosphothreonine-containing proteins by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphothreonine Mouse mAb (14B3) (Cat. No. 525286) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphothreonine (1E11) (mouse) (Cat. No. 525287), and Phosphothreonine Detection Kit (Cat. No. 525288)
      References
      Product Information
      FormLyophilized
      Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and 150 µl control lysate lyophilized from 2x PBS, PEG, sucrose, 10 mM NaF.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vandate-treated A431 cells)
      Application CommentsFor immunoblotting with the positive control, use 5 µl (chemiluminescence) to 20 µl (colorimetric) per mini-gel lane. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena mixture of synthetic phosphopeptides containing phosphothreonine, conjugated to KLH
      Clone14B3
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml H₂O (15 min, RT). Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      525286 0

      Documentation

      PhosphoDetect™ Anti-Phosphothreonine Mouse mAb (14B3) Analysenzertifikate

      TitelChargennummer
      525286
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-February-2011 JSW
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vandate-treated A431 cells)
      Application Data
      Detection of phosphothreonine-containing proteins by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphothreonine Mouse mAb (14B3) (Cat. No. 525286) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphothreonine (1E11) (mouse) (Cat. No. 525287), and Phosphothreonine Detection Kit (Cat. No. 525288)
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes phosphothreonine-containing proteins. Supplied with a positive control consisting of phosphoproteins isolated from rabbit muscle by affinity chromatography using Fe3+-IDA-sepharose.
      HostMouse
      Immunogena mixture of synthetic phosphopeptides containing phosphothreonine, conjugated to KLH
      Clone14B3
      IsotypeIgG₁
      Speciesa broad range of species
      FormLyophilized
      Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and 150 µl control lysate lyophilized from 2x PBS, PEG, sucrose, 10 mM NaF.
      Preservative≤0.1% sodium azide
      CommentsFor immunoblotting with the positive control, use 5 µl (chemiluminescence) to 20 µl (colorimetric) per mini-gel lane. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml H₂O (15 min, RT). Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Toxicity Standard Handling