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DR1027 PhosphoDetect™ Anti-MDM2 (pSer¹⁶⁶) Rabbit pAb

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Synonyme: Ant-Murine Double Minute Chromosome-2

DR1027
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~90 kDa (apparent MW) MDM2 protein phosphorylated at Ser166 in IGF-1-treated MCF-7 cells.
      Catalogue NumberDR1027
      Brand Family Calbiochem®
      SynonymsAnt-Murine Double Minute Chromosome-2
      Application Data
      Detection of human MDM2, phosphorylated at Ser166, by immunoblotting. Samples: Whole cell lysate from MCF7 cells treated with IGF-1 for the indicated times. Primary antibody: PhosphoDetect™ Anti-MDM2 (pSer166) Rabbit pAb (Cat. No. DR1027) (1:1000). Detection: chemiluminescence.
      References
      ReferencesMayo, L.D., and Donner, D.B. 2001. Proc. Natl. Acad. Sci. USA 98, 11598.
      Zhou, B.P., et al. 2001. Nat. Cell. Biol 3, 973.
      Grossman, S.R., et al. 1998. Mol. Cell 2, 405.
      Haupt, Y., et al. 1997. Nature 387, 296.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlMCF7 cells treated with IGF-1
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsDoes not recognize unphosphorylated MDM2. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁶⁶ phosphorylation site of human MDM2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      DR1027 0

      Documentation

      PhosphoDetect™ Anti-MDM2 (pSer¹⁶⁶) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      DR1027

      Literatur

      Übersicht
      Mayo, L.D., and Donner, D.B. 2001. Proc. Natl. Acad. Sci. USA 98, 11598.
      Zhou, B.P., et al. 2001. Nat. Cell. Biol 3, 973.
      Grossman, S.R., et al. 1998. Mol. Cell 2, 405.
      Haupt, Y., et al. 1997. Nature 387, 296.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnt-Murine Double Minute Chromosome-2
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of human MDM2, phosphorylated at Ser166, by immunoblotting. Samples: Whole cell lysate from MCF7 cells treated with IGF-1 for the indicated times. Primary antibody: PhosphoDetect™ Anti-MDM2 (pSer166) Rabbit pAb (Cat. No. DR1027) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein phosphorylated at Ser166.
      BackgroundMDM2 is an ubiquitin ligase that plays a central role in the regulation of p53. MDM2 is phosphorylated on Ser166 and Ser186 by ATM. This phosphorylation of MDM2 increases its interactions with p300 allowing the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, which contributes to an increase in the degradation of p53.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁶⁶ phosphorylation site of human MDM2
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlMCF7 cells treated with IGF-1
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsDoes not recognize unphosphorylated MDM2. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Toxicity Standard Handling
      ReferencesMayo, L.D., and Donner, D.B. 2001. Proc. Natl. Acad. Sci. USA 98, 11598.
      Zhou, B.P., et al. 2001. Nat. Cell. Biol 3, 973.
      Grossman, S.R., et al. 1998. Mol. Cell 2, 405.
      Haupt, Y., et al. 1997. Nature 387, 296.