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516726 Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications.

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516726
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516726-1SET
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      Description
      OverviewA 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.
      Catalogue Number516726
      Brand Family Calbiochem®
      References
      Product Information
      FormTwo-layer colorless liquid
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥99% by titration (phenol); >99% by HPLC (chloroform)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 23/24/25-34-40

      Toxic by inhalation, in contact with skin and if swallowed.
      Causes burns.
      Limited evidence of a carcinogenic effect.
      S PhraseS: 26-27-36/37/39-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Take off immediately all contaminated clothing.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing equilibration, refrigerate (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      516726-1SET 07790788050993

      Documentation

      Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem Analysenzertifikate

      TitelChargennummer
      516726
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-January-2010 RFH
      DescriptionA 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Prior to equilibration with the Tris-based equilibration buffer (pH ~6.7), the mix is ideal for extraction of proteins and lipids for RNA purification. Following equilibration with the Tris-based equilibration buffer (pH ~8.0), is ideal for extraction of proteins and lipids for DNA purification. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.
      FormTwo-layer colorless liquid
      Recommended reaction conditions
      Equilibration To equilibrate add the entire bottle (26 ml) of Equilibration Buffer, mix and allow to stand for 1 h before use.
      Purity≥99% by titration (phenol); >99% by HPLC (chloroform)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing equilibration, refrigerate (4°C).
      Toxicity Toxic