Millipore Sigma Vibrant Logo

539568 PP2B, Human, Recombinant, E. coli

PP2B, Human, Recombinant, E. coli: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: CaN, Protein Phosphatase 2B, Calcineurin

View Products on Sigmaaldrich.com
539568
Preis & Verfügbarkeit

Übersicht

Replacement Information

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
539568-5000U
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Kst.-Ampulle 5000 u
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      Description
      OverviewRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
      Catalogue Number539568
      Brand Family Calbiochem®
      SynonymsCaN, Protein Phosphatase 2B, Calcineurin
      References
      ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; <a href ="/Products/ProductDisplay.asp→catNO=207008">Cat. No. 207008</a>) in the presence of calmodulin per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
      Quality LevelMQ100
      Applications
      Biological Information
      PurityTwo distinct bands by SDS-PAGE
      Specific Activity≥300,000 units/mg
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      539568-5000U 04055977194685

      Documentation

      PP2B, Human, Recombinant, E. coli SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      PP2B, Human, Recombinant, E. coli Analysenzertifikate

      TitelChargennummer
      539568

      Literatur

      Übersicht
      Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.

      Broschüre

      Titel
      Protein Phosphatases Technical Bulletin
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2011 JSW
      SynonymsCaN, Protein Phosphatase 2B, Calcineurin
      DescriptionRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
      PurityTwo distinct bands by SDS-PAGE
      Specific activity≥300,000 units/mg
      Activity≥50,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
      Toxicity Standard Handling
      ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.