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539620 PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem

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Synonyme: Protein Phosphatase 2A Inhibitor II, I₂PP2A

Primary Target: PP2A
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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human PP2AI I2 fused to GST and expressed in E. coli. Inhibits protein phosphatase 2A (PP2A) potently with Ki ~ 25-30 nM with myelin basic protein, histone H1, and other substrates of PP2A but not with casein as a substrate. Stimulates PP1. Prevents inhibition of E-Cdk-2 by p21Cip1. Associates with B cyclin. Regulates histone binding to DNA, transcriptional activity and chromatin condensation. Undergoes phosphorylation in cells. Highly expressed in some cancers. Associates with HRX leukemia-associated fusion proteins. Occurs as fusion protein in acute undifferentiated leukemia. Does not inhibit PP1, PP2B, PP2C, or pyruvate dehydrogenase.
      Catalogue Number539620
      Brand Family Calbiochem®
      SynonymsProtein Phosphatase 2A Inhibitor II, I₂PP2A
      References
      ReferencesLi, M., et al. 1996. Biochemistry 35, 6998.
      Li, M., et al. 1995. Biochemistry 34, 1988.
      Product Information
      ATP CompetitiveN
      FormLiquid
      FormulationIn 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetPP2A
      Primary Target K<sub>i</sub>~ 25-30 nM
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      539620 0

      Documentation

      PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem Analysenzertifikate

      TitelChargennummer
      539620

      Literatur

      Übersicht
      Li, M., et al. 1996. Biochemistry 35, 6998.
      Li, M., et al. 1995. Biochemistry 34, 1988.

      Broschüre

      Titel
      Protein Phosphatases Technical Bulletin
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-June-2011 JSW
      SynonymsProtein Phosphatase 2A Inhibitor II, I₂PP2A
      DescriptionRecombinant, human PP2AI I2 fused to GST and expressed in E. coli. Inhibits PP2A with myelin basic protein, histone H1 or phosphorylase as substrates, but not with casein as the substrate. Inhibits PP2A in a noncompetitive manner (Ki = ~25-30 nM with myelin basic protein as the substrate). Does not inhibit protein phosphatase 1, protein phosphatase 2B, protein phosphatase 2C, or pyruvate dehydrogenase phosphatase.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesLi, M., et al. 1996. Biochemistry 35, 6998.
      Li, M., et al. 1995. Biochemistry 34, 1988.