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539494 PKC, Rat Brain

PKC, Rat Brain: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Protein Kinase C

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      Übersicht

      Replacement Information
      Description
      OverviewNative PKC from rat brain predominantly containing the conventional PKC isoforms, α, β1, β2, and γ. A ubiquitous phospholipid-dependent enzyme involved in signal transduction associated with cell proliferation and differentiation. At least 10 PKC isoforms are reported to exist, which are classified as conventional (α, β, γ), novel (δ, e, η, θ, µ), and atypical (ξ, λ) isoforms.
      M.W. 76,000-82,000.
      Catalogue Number539494
      Brand Family Calbiochem®
      SynonymsProtein Kinase C
      References
      ReferencesChang, E.Y., et al. 1997. J. Immunol. 159, 2624.
      Hanks, S.K. and Hunter, T. 1995. FASEB J. 9, 576.
      Nishizuka, Y. 1995. FASEB J. 9, 484.
      Hannun, Y.A. and Bell, R.M. 1990. J. Biol. Chem. 265, 2962.
      Bell, R.M., et al. 1986. Methods Enzymol. 124, 353.
      Kikkawa, U., et al. 1986. Biochem. Biophys. Res. Commun. 135, 636.
      Kikkawa, U., et al. 1982. J. Biol. Chem. 257, 13341.
      Product Information
      CAS number141436-78-4
      Activity42 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to histone III-S per min at 22°C, pH 7.4.
      EC number2.7.1.37
      FormLiquid
      FormulationIn 100 mM NaCl, 20 mM Tris-HCl, 1 mM DTT, 500 µM EDTA, 500 µM EGTA, 10% glycerol, pH 7.5.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥1000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Stable for up to 4 days at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      539494 0

      Documentation

      PKC, Rat Brain Analysenzertifikate

      TitelChargennummer
      539494

      Literatur

      Übersicht
      Chang, E.Y., et al. 1997. J. Immunol. 159, 2624.
      Hanks, S.K. and Hunter, T. 1995. FASEB J. 9, 576.
      Nishizuka, Y. 1995. FASEB J. 9, 484.
      Hannun, Y.A. and Bell, R.M. 1990. J. Biol. Chem. 265, 2962.
      Bell, R.M., et al. 1986. Methods Enzymol. 124, 353.
      Kikkawa, U., et al. 1986. Biochem. Biophys. Res. Commun. 135, 636.
      Kikkawa, U., et al. 1982. J. Biol. Chem. 257, 13341.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-June-2009 RFH
      SynonymsProtein Kinase C
      DescriptionNative PKC from rat brain predominantly containing the conventional PKC isoforms, α, β1, β2, and γ. A ubiquitous phospholipid-dependent enzyme involved in signal transduction associated with cell proliferation and differentiation. At least 10 PKC isoforms are reported to exist, which are classified as conventional (α, β, γ), novel (δ, e, η, θ, µ), and atypical (ξ, λ) isoforms.
      FormLiquid
      FormulationIn 100 mM NaCl, 20 mM Tris-HCl, 1 mM DTT, 500 µM EDTA, 500 µM EGTA, 10% glycerol, pH 7.5.
      CAS number141436-78-4
      EC number2.7.1.37
      Purity≥95% by SDS-PAGE
      Specific activity≥1000 units/mg protein
      Activity42 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to histone III-S per min at 22°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Stable for up to 4 days at 4°C.
      Toxicity Standard Handling
      ReferencesChang, E.Y., et al. 1997. J. Immunol. 159, 2624.
      Hanks, S.K. and Hunter, T. 1995. FASEB J. 9, 576.
      Nishizuka, Y. 1995. FASEB J. 9, 484.
      Hannun, Y.A. and Bell, R.M. 1990. J. Biol. Chem. 265, 2962.
      Bell, R.M., et al. 1986. Methods Enzymol. 124, 353.
      Kikkawa, U., et al. 1986. Biochem. Biophys. Res. Commun. 135, 636.
      Kikkawa, U., et al. 1982. J. Biol. Chem. 257, 13341.