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324881 PERK, GST-Fusion, Human, Recombinant, E. coli

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324881
  
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      Übersicht

      Description
      Overview

      This product has been discontinued.



      Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening.

      Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.
      Catalogue Number324881
      Brand Family Calbiochem®
      SynonymsEIF2AK3 PKR
      Application Data
      The activity of PERK was measured as outlined in the Recommended Reaction Conditions.
      References
      ReferencesBaltzis, D., et al. 2007. J Biol Chem. 43, 31675.
      Koumenis, C., et al. 2002. Mol Cell Biol. 21, 7405.


      Product Information
      Unit of DefinitionKinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific ActivityPlease refer to the lot-specific Certificate of Analysis
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Bestellnummer GTIN
      324881 0