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512777 PBSF/SDF-1β, Human, Recombinant, E. coli

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: rhPBSF/SDF-1β

512777
  
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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, human PBSF/SDF-1β expressed in E. coli. Supports the proliferation of a stromal cell-dependent pre-B cell line. Found to be a chemoattractant for T lymphocytes and monocytes but not neutrophils. Recently shown to be a ligand for the CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte-tropic HIV-1 strains.
      Catalogue Number512777
      Brand Family Calbiochem®
      SynonymsrhPBSF/SDF-1β
      References
      ReferencesBleul, C., et al. 1996. Nature 382, 829.
      Oberlin, E., et al. 1996. Nature 382, 833.
      Nagasawa, T., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2305.
      Tashiro, K., et al. 1993. Science 261, 600.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg PBSF/SDF-1β.
      Applications
      Biological Information
      Biological activityED₅₀ = 50-100 ng/ml as measured by its ability to chemoattract human T lymphocytes cultured in the presence of IL-2
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤100 pg/µg PBSF/SDF-1β
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      512777 0

      Documentation

      PBSF/SDF-1β, Human, Recombinant, E. coli Analysenzertifikate

      TitelChargennummer
      512777

      Literatur

      Übersicht
      Bleul, C., et al. 1996. Nature 382, 829.
      Oberlin, E., et al. 1996. Nature 382, 833.
      Nagasawa, T., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2305.
      Tashiro, K., et al. 1993. Science 261, 600.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-August-2008 RFH
      SynonymsrhPBSF/SDF-1β
      DescriptionRecombinant, human PBSF/SDF-1β expressed in E. coli. Supports the proliferation of a stromal cell-dependent pre-B cell line. Found to be a chemoattractant for T-lymphocytes and monocytes but not neutrophils. Recently shown to be a ligand for the CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte tropic HIV-1 strains.
      FormLyophilized
      FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg PBSF/SDF-1β.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤100 pg/µg PBSF/SDF-1β
      Biological activityED₅₀ = 50-100 ng/ml as measured by its ability to chemoattract human T lymphocytes cultured in the presence of IL-2
      SolubilityReconstitute to a concentration ≥50 µg/ml with sterile PBS containing as least 0.1% HSA or BSA.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
      Toxicity Standard Handling
      ReferencesBleul, C., et al. 1996. Nature 382, 829.
      Oberlin, E., et al. 1996. Nature 382, 833.
      Nagasawa, T., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 2305.
      Tashiro, K., et al. 1993. Science 261, 600.