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2740-OP OmniPur® Bradford Method Protein Assay Kit - Calbiochem

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2740-OP
  
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      Replacement Information
      Description
      OverviewContains: Bradford Reagent, 0.15 M Sodium Chloride, and 0.5 mg/ml BSA Solution.

      Specifications:
      BSA Solution BSA Content: 0.45-0.55mg/ml
      BSA Solution Protease: None detected
      Bradford Reagent Pattern: Passes test
      Bradford Reagent Protease: None detected
      Sodium Chloride Solution Concentration: 130-170 mmol/l





      Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
      Catalogue Number2740-OP
      Brand Family Calbiochem®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      2740-OP 0

      Documentation

      OmniPur® Bradford Method Protein Assay Kit - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      OmniPur® Bradford Method Protein Assay Kit - Calbiochem Analysenzertifikate

      TitelChargennummer
      2740-OP
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-September-2012 JSW
      DescriptionThe Bradford method is a rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.
      Recommended reaction conditions

      Materials Supplied
      • 1 liter of Bradford reagent
      • 1.5 ml of BSA
      • 200 ml of Sodium Chloride solution

      Protocol
      1. Into 4 separate microcentrifuge tubes, aliquot 5, 10, 15 and 20 µl of 0.5 mg/ml BSA solution. Bring the volume of each to 100 µl with 0.15N NaCl.
      2. Into 1 tube, aliquot 100 µl 0.15N NaCl. This will serve as a blank.
      3. Add to each tube, 1 ml Bradford Reagent and vortex. Allow to stand at room temperature for 2 minutes.
      4. Determine A 595 nm using a 1 ml microcuvette. Generate a standard curve by plotting absorbance at 595 nm versus protein concentration.
      5. For the unknown sample, repeat steps 1-4 using the unknown in place of the BSA. Plot the A 595 nm and use the standard curve as a reference to determine the concentration of the unknown sample.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze