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SCC136 OP9-DL1 hDL-1-Expressing mOP9 Cell Line

OP9 bone marrow stromal OP9-DL1 cells support the differentiation of embryonic or hematopoietic stem cells from fetal liver or bone marrow into T lymphocytes. Holmes et. al., has provided a detailed protocol to differentiate embryonic or hematopoietic stem cells into T lymphocytes using the OP9-DL1 system. The OP9-DL1 cell line is an important tool for the exploration of the role of Notch signaling in cellular differentiation and genesis of inflammatory disease states.

SCC136
≥1X10<sup>6</sup> cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC136
      DescriptionOP9-DL1 hDL-1-Expressing mOP9 Cell Line
      Alternate Names
      • OP9-DL1
      • OP9-DL1 cell line
      • DL1 cell line
      • Delta-like 1 cell line
      • Notch ligand cell line
      Background InformationOP9-DL1 is a bone marrow-derived stromal cell line stably expressing the Notch ligand Delta like I (DL1) ectopically.
      Notch signaling controls multiple cell fate decisions. It is initiated via engagement of the Notch receptor with its ligands (Jagged or Delta-like family members). This is followed by cleavage of the intracellular domain of Notch and its subsequent translocation into the nucleus, where it binds with the transcription factor CBF-1/RBPJ and activates transcription of various downstream target genes. Upregulation of Notch ligands is a factor in chronic inflammatory diseases including pancreatitis and diabetic nephropathy. Several studies have implicated the role of Notch signaling in early T-cell lineage commitments, as it promotes development of T-cells with αβ+ T-Cell Receptors (TCRs) at the expense of γδ+ TCRs 3. Bone marrow progenitor cells expressing constitutively active Notch have been shown to develop into CD4 and CD8 double-positive T cells. This signaling pathway also plays an important role in the development of CD4+ or CD8+ single positive cells from CD4 and CD8 double-positive precursor thymocytes.

      OP9 bone marrow stromal cells support the differentiation of hematopoietic progenitor cells (HPCs) into multiple lineages including B-cells, but not to T-cells. This is mainly because they do not express Delta-like 1 or Delta-like 4 notch ligands inherently. OP9 cells were therefore infected with a MiGR1 retroviral vector engineered to express the human Delta-like-1 gene and green fluorescent protein (GFP). The OP9-DL1 cells were then sorted for GFP expression.

      Source
      GMO. OP9-DL1 cells were genetically modified from stromal cells derived from mouse bone marrow.

      References
      1. Immunity 2002, 17(6): 749-756.
      2. J Orthop Sci. 2005, 10(6): 589-594.
      3. Curr Opin Immunol. 2007, 19(2): 163-168.
      4. Cold Spring Harb Protoc. 2009, 2009(2): pdb.prot5156.
      References
      Product Information
      Components
      • ≥1x106 viable OP9-DL1 hDL-1-Expressing mOP9 Cells: (Catalog No. SCC136). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationOP9 bone marrow stromal OP9-DL1 cells support the differentiation of embryonic or hematopoietic stem cells from fetal liver or bone marrow into T lymphocytes. Holmes et. al., has provided a detailed protocol to differentiate embryonic or hematopoietic stem cells into T lymphocytes using the OP9-DL1 system. The OP9-DL1 cell line is an important tool for the exploration of the role of Notch signaling in cellular differentiation and genesis of inflammatory disease states.
      Key Applications
      • Cell Differentiation
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostMouse
      Cell Line Type
      • Stromal Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsOP9-DL1 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
      Packaging Information
      Material Size≥1X106 cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SCC136 04065269797913