Natrix® Q Chromatography Membrane
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| Biopharmaceutical Manufacturing > Downstream Processing > Chromatography > Membrane Chromatography |
| Primary characteristics of Natrix® Q chromatography membrane | |
| Hydrogel | Porous polyacrylamide |
| Functional group | Amidopropyl trimethylammonium chloride (quaternary amine) |
| Typical flow rate | 5-25 MV/min |
| Nominal pore size | 0.40 µm |
| BSA binding capacity | > 200 mg/mL |
| DNA binding capacity | > 20 mg/mL |
| Chemical compatibility | Compatible with most buffers and solvents commonly used in chromatographic biomolecule purification processes (see the Validation Guide for complete and detailed information) |
| Incompatible chemicals | Hypochlorite (1%); SDS (1%) |
| Shipment conditions | Dry, ready to use (free of preservatives or wetting agents) |
| Storage conditions | Store at room temperature prior to use |
| Natrix® Q Chromatography Membrane Device Properties | ||||
| Recon Mini | Pilot | Process 150 | Process 600 | |
| Nominal membrane volume (mL) | 0.2 | 15 | 115 | 460 |
| Minimum standardized BSA binding capacity (g) | 0.04 | 3 | 23 | 92 |
| mAb nominal polishing @ 10 kg/L load (g)* | 2 | 150 | 1150 | 4600 |
| Membrane configuration | Flat Sheet | Pleated | ||
| Flow rate range** | 1 - 5 mL/min | 75 – 375 mL/min | 0.6 – 3 L/min | 2 – 10 L/min |
| Intended use | Scaled down laboratory model to screen and fine-tune parameters. | Intermediate scale adsorber, intended to verify and adjust operating parameters. Pilot may be used for small-scale clinical and commercial manufacturing. | Process scale adsorbers designed for full-scale GMP manufacturing of proteins | |
| *Based on typical process streams and loading up to 10 kg mAb/L membrane. Loading capacity is not limited to 10 kg mAb/L and depends on the total impurity content. **Typical flow rate range is based on 5-25 membrane volumes/minute. Specific flow rates can be determined to accommodate process requirements (e.g. maximum back pressure, improved process time, etc.). |
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