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481418 NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: NEMO Ubiquitin Binding Domain Inhibitor

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      Übersicht

      Replacement Information

      Key Spec Table

      Empirical Formula
      C₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
      Description
      Overview

      This product has been discontinued.



      A cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
      Catalogue Number481418
      Brand Family Calbiochem®
      SynonymsNEMO Ubiquitin Binding Domain Inhibitor
      References
      ReferencesChiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.
      Product Information
      FormWhite powder
      Hill FormulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
      Chemical formulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Peptide SequenceRQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      481418 0

      Documentation

      NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem Analysenzertifikate

      TitelChargennummer
      481418

      Literatur

      Übersicht
      Chiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-August-2012 JSW
      SynonymsNEMO Ubiquitin Binding Domain Inhibitor
      DescriptionA cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
      FormWhite powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
      Peptide SequenceRQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
      Purity≥95% by HPLC
      SolubilityH₂O (50 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesChiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.