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Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
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HSTCMAG384-PX21
MilliporeMILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel - Immunology Multiplex Assay
This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay.
More>>This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel - Immunology Multiplex Assay
Overview
EMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel is t to be used for the simultaneous quantification of these analytes in serum, plasma, or tissue/cell culture supernatant: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP1alpha/CCL3, MIP1beta/CCL4, MIP-3alpha/CCL20 and TNFalpha.
MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
EMD Millipore’s MILLIPLEX MAP 384-well Human High Sensitivity T Cell Premixed 21-Plex Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information
Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.
Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
The 384-well Human High Sensitivity T Cell Panel is the 384-well version of the current 96-well Human High Sensitivity T Cell Panel, and it exceeds expectations in terms of throughput, sensitivity (see table) and sample detectability. Data obtained from the High Sensitivity T Cell Panel will correlate with data for the respective cytokines in the Human Cytokine/Chemokine Panels I, II and III, furthering your ability to measure specific cytokine response in both normal and disease states.
This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay.
Key Applications
Multiplexing
Application Notes
• This is an overnight incubation. • Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. • This kit may be used in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
Human
Analytes Available
Fractalkine
GM-CSF
IFNγ
IL-1β
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12 (p70)
IL-13
IL-17A
IL-21
IL-23
ITAC
MIP-1α
MIP-1β
MIP-3α
TNF-α
Cross Reactivity
There is no detectable cross-reactivity within the panel.
Accuracy
79% – 108%
Physicochemical Information
Sensitivity
Please see kit protocol for individual assay sensitivities.
Linearity of Dilution
Please contact Technical Service for information.
Standard Curve Range
Fractalkine: 18.3 – 75,000 pg/mL
GM-CSF: 1.22 – 5,000 pg/mL
IFNγ: 0.61 – 2,500 pg/mL
IL-1β: 0.49 – 2,000 pg/mL
IL-2: 0.49 – 2,000 pg/mL
IL-4: 1.83 – 7,500 pg/mL
IL-5: 0.49 – 2,000 pg/mL
IL-6: 0.18 – 750 pg/mL
IL-7: 0.37 – 1,500 pg/mL
IL-8: 0.31 – 1,250 pg/mL
IL-10: 1.46 – 6,000 pg/mL
IL-12 (p70): 0.49 – 2,000 pg/mL
IL-13: 0.24 – 1,000 pg/mL
IL-17A: 0.73 – 3,000 pg/mL
IL-21: 0.24 – 1,000 pg/mL
IL-23: 7.93 – 32,500 pg/mL
ITAC: 1.46 – 6,000 pg/mL
MIP-1α: 0.31 – 1,250 pg/mL
MIP-1β: 0.92 – 3,750 pg/mL
MIP-3α: 0.61 – 2,500 pg/mL
TNFα: 0.43 – 1,750 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Recommended storage for kit components is 2 - 8°C.
