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WB39 MCF7 Cell Pellet

MCF7 Cell Pellet: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
WB39
  
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      Übersicht

      Description
      OverviewA human breast adenocarcinoma cell line with an epithelial cell morphology that was originally isolated from a pleural effusion. Useful as a positive control Western blotting of various proteins, such as estrogen receptors and the insulin-like growth factor binding proteins (IGFBP) BP-2, BP-4, and BP-5.
      Catalogue NumberWB39
      Brand Family Calbiochem®
      Product Information
      FormFlash-frozen cell pellet
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application CommentsSuggested Preparation for Use

      Laemmli Sample Buffer (SDS-PAGE)
      Resuspend the frozen cell pellet by adding 1.0 ml of Laemmli sample buffer (containing fresh 2-mercaptoethanol, 2-ME, at a final concentration of 500 mM) to the vial containing the cell pellet followed by gentle vortexing. (Alternatively dithiothreitol (DTT) to a final concentration of 100 mM may be substituted for the 2-ME). Heat the sample for 5 min at 90°C. Apply 15-25 µl to each lane of the gel. This is a starting amount, as actual amounts for optimal protein detection need to be experimentally determined for every antibody. Freeze the unused material in 25 µl aliquots and store at -70°C. (Before using the frozen samples, fresh 2-ME or DTT should be added and the sample heated for 5 min at 90°C).

      Lane's Sample Buffer (IP/Immunoblot applications)
      Resuspend the frozen pellet by adding 1.0 ml of Lane's lysis buffer (150 mM NaCl, 50 mM Tris-HCl, pH 8.0, 5 mM EDTA, and 1% NP40) to the vial containing the cell pellet followed by gentle vortexing. Fresh 2-mercaptoethanol, 2-ME, must be added to the vial at a concentration of 500 mM prior to heating the sample for 5 min at 90°C. (Alternatively dithiothreitol (DTT) to a final concentration of 100 mM may be substituted for the 2-ME). Apply 15-25 µl to each lane of the gel. This is a starting amount, as actual amounts for optimal protein detection need to be experimentally determined for every antibody. Freeze the unused material in 25 µl aliquots and store at -70°C. (Before using the frozen samples, fresh 2-ME or DTT should be added and the sample heated for 5 min at 90°C).
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsStore at -70°C until lysed as described below. Following resuspension, store unused material in 25 µl aliquots at -70°C until needed. Do not expose to repeated cycles of freezing and thawing.
      Global Trade Item Number
      Bestellnummer GTIN
      WB39 0