Millipore Sigma Vibrant Logo

440202 LY 294002 - CAS 154447-36-6 - Calbiochem

LY294002, CAS 154447-36-6, is a cell-permeable, potent, reversible, and specific inhibitor of PI 3-kinase ((IC₅₀ = 1.4 µM). Acts on the ATP-binding site.

LY 294002 - CAS 154447-36-6 - Calbiochem: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, BRD2 Inhibitor IV, BRD3 Inhibitor III, BRD4 Inhibitor IV

Primary Target: Phosphatidylinositol 3-kinase
View Products on Sigmaaldrich.com
440202
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

CAS #Empirical Formula
154447-36-6C₁₉H₁₇NO₃

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
440202-10MG
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Kst.-Ampulle 10 mg
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      440202-25MG
      Verfügbarkeit wird abgerufen...
      —
      Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
      Lieferbar 
      Produkt wurde eingestellt
      Begrenzter Lagerbestand
      Bestätigung der Verfügbarkeit erforderlich
        Restmenge: Angebot folgt
          Restmenge: Angebot folgt
          Bitte erfragen
          Kontakt zum Kundenservice
          Contact Customer Service

          		 Kst.-Ampulle 25 mg
          Preis wird abgerufen...
          Preis nicht abrufbar
          Die Mindestmenge muss ein Vielfaches sein von
          Maximum Quantity is
          Bei Bestätigung Weitere Informationen
          Sie haben () gespart
           
          —
          Bitte erfragen
          		 —
          440202-5MG
          Verfügbarkeit wird abgerufen...
          —
          Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
          Lieferbar 
          Produkt wurde eingestellt
          Begrenzter Lagerbestand
          Bestätigung der Verfügbarkeit erforderlich
            Restmenge: Angebot folgt
              Restmenge: Angebot folgt
              Bitte erfragen
              Kontakt zum Kundenservice
              Contact Customer Service

              		 Kst.-Ampulle 5 mg
              Preis wird abgerufen...
              Preis nicht abrufbar
              Die Mindestmenge muss ein Vielfaches sein von
              Maximum Quantity is
              Bei Bestätigung Weitere Informationen
              Sie haben () gespart
               
              —
              Bitte erfragen
              		 —
              Description
              OverviewA cell-permeable, potent, reversible and specific phosphatidylinositol 3-kinase inhibitor (IC50 = 1.4 µM) that acts on the ATP-binding site of the enzyme. Also inhibits nonhomologous DNA end-joining (NHEJ) activity of the 460 kDa phosphatidylinositol 3-like kinase DNA-PKcs, which is the catalytic subunit of DNA-activated protein kinase. Does not affect the activities of EGF receptor kinase, MAP kinase, PKC, Pl 4-kinase, S6 kinase, and c-Src even at 50 µM. Promotes the nuclear accumulation of active GSK-3b. Reported to suppress the proliferation of murine embryonic stem cells. Blocks the proliferation of cultured rabbit aortic smooth muscle cells without inducing apoptosis. Shown to bind to the same site as I-BET (Cat. No. 40101050 ~ 10 µM). A 10 mM (1 mg/325 µl) solution of LY 294002 (Cat. No. 440204) in DMSO is also available.
              Catalogue Number440202
              Brand Family Calbiochem®
              Synonyms2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, BRD2 Inhibitor IV, BRD3 Inhibitor III, BRD4 Inhibitor IV
              References
              ReferencesDittman, A., et al. 2013. ACS Chem. Biol. 9, 495.
              Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
              Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
              Baumann, P., and West, S.C. 1998. Proc. Natl. Acad. Sci. USA 95, 14066.
              Cardone, M.H., et al. 1998. Science 282, 1318.
              Vlahos, C.J., et al. 1995. J. Immunol. 154, 2413.
              Yano, H., et al. 1995. Biochem. J. 312, 145.
              Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.



              Selected Citations
              Lee, J., et al. 2009. Cell Stem Cell 5, 76.
              Product Information
              CAS number154447-36-6
              ATP CompetitiveY
              FormOff-white solid
              Hill FormulaC₁₉H₁₇NO₃
              Chemical formulaC₁₉H₁₇NO₃
              ReversibleY
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              Biological Information
              Primary TargetPhosphatidylinositol 3-kinase
              Primary Target IC<sub>50</sub>1.4 µM against phosphatidylinositol 3-kinase
              Purity≥98% by HPLC
              Physicochemical Information
              Cell permeableY
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage -20°C
              Protect from Light Protect from light
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Bestellnummer GTIN
              440202-10MG 04055977186666
              440202-25MG 04055977186673
              440202-5MG 07790788050290

              Documentation

              LY 294002 - CAS 154447-36-6 - Calbiochem SDB

              Titel

              Sicherheitsdatenblatt (SDB) 

              LY 294002 - CAS 154447-36-6 - Calbiochem Analysenzertifikate

              TitelChargennummer
              440202

              Literatur

              Übersicht
              Dittman, A., et al. 2013. ACS Chem. Biol. 9, 495.
              Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
              Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
              Baumann, P., and West, S.C. 1998. Proc. Natl. Acad. Sci. USA 95, 14066.
              Cardone, M.H., et al. 1998. Science 282, 1318.
              Vlahos, C.J., et al. 1995. J. Immunol. 154, 2413.
              Yano, H., et al. 1995. Biochem. J. 312, 145.
              Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.



              Selected Citations
              Lee, J., et al. 2009. Cell Stem Cell 5, 76.

              Broschüre

              Titel
              An Introduction to Inhibitors and Their Biological Applications - 1st Edition
              Bulk Product Guide

              Technische Informationen

              Titel
              JAK/STAT Signaling Research Focus

              Datenblatt

              Titel
              Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

              Posters

              Titel
              Human Kinome & InhibitorSelect™ Libraries

              Literaturstellen

              Titel
            • Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
            • Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
            • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
            • Satoshi Fukumoto, et al. (2006) Laminin 5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. Journal of Biological Chemistry 281, 5008-5016.
            • Ana Olivera, et al. (2006) IgE-dependent activation of sphingosine kinase 1 and 2 and secretion of sphingosine-1-phosphate requires Fyn kinase and contributes to mast cell responses. Journal of Biological Chemistry 281, 2515-2525.
            • Qian Yang, et al. (2006) TSC1/TSC2 and Rheb have different effects on TORC1 and TORC2 activity. Proceedings of the National Academy of Sciences (USA) 103, 6811-6816.
            • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
            • Huanyu Dou, et al. (2005) Neuroprotective mechanisms of lithium in murine human immunodeficiency virus-1 encephalitis. Journal of Neuroscience 25, 8375-8385.
            • Julie Lotharius, et al. (2005) Progressive degeneration of human mesencephalic neuron-derived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed lineage kinase pathway. Journal of Neuroscience 25, 6329-6342.
            • Meenalakshmi M. Mariappan, M.M., et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
            • Michael Tscharntke, et al. (2005) Ras-induced spreading and wound closure in human epidermal keratinocytes. The FASEB Journal in press,.
            • Junji Tsurutani, et al. (2005) Inhibition of the phosphatidylinositol 3-kinase/Akt/Mammalian target of rapamycin pathway but no the MEK/ERK Pathway attentuates laminin-meidated small cell lunch cancer cellular survival and resistance to Imatinib mesylate or chemolyte.. Cancer Research 65, 8423-8432.
            • Xinbo Liao, et al. (2003) Androgen stimulates matrix metalloproteinase-2 expression in human prostate cancer. Endocrinology 144, 1656-1663.
              		•
              Datenblatt

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision28-February-2012 RFH
              Synonyms2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, BRD2 Inhibitor IV, BRD3 Inhibitor III, BRD4 Inhibitor IV
              DescriptionA cell-permeable, potent, reversible, and specific phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor that acts on the ATP-binding site of the enzyme (IC50 = 1.4 µM). Also inhibits nonhomologous DNA end-joining (NHED) in the 460 kDa phosphatidylinositol 3-like kinase DNA-PKcs, which is the catalytic subunit of DNA-activated protein kinase. Does not affect the activity of EGF receptor kinase, MAP kinase, PKC, PI 4-kinase, S6 kinase, and c-src at 50 µM. Promotes the nuclear accumulation of active GSK-3b. Reported to suppress the proliferation of murine embryonic stem cells. Blocks the proliferation of cultured rabbit aortic smooth muscle cells without inducing apoptosis. Reported to inhibit histamine secretion in RBL-2H3 cells (IC50 = 5 µM).
              FormOff-white solid
              CAS number154447-36-6
              Chemical formulaC₁₉H₁₇NO₃
              Structure formulaStructure formula
              Purity≥98% by HPLC
              SolubilityDMSO (20 mg/ml) or Ethanol. Slight warming may be required to achieve complete solubilization.
              Storage Protect from light
              -20°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
              Toxicity Standard Handling
              ReferencesDittman, A., et al. 2013. ACS Chem. Biol. 9, 495.
              Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
              Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
              Baumann, P., and West, S.C. 1998. Proc. Natl. Acad. Sci. USA 95, 14066.
              Cardone, M.H., et al. 1998. Science 282, 1318.
              Vlahos, C.J., et al. 1995. J. Immunol. 154, 2413.
              Yano, H., et al. 1995. Biochem. J. 312, 145.
              Vlahos, C.J., et al. 1994. J. Biol. Chem. 269, 5241.



              Selected Citations
              Lee, J., et al. 2009. Cell Stem Cell 5, 76.
              Citation
            • Bechard, M., and Dalton. S. 2009. Mol. Cell. Biol. 29, 2092.
            • Lianguzova, M.S. et al. 2007. Cell Biol. Int. 31, 330.
            • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
            • Satoshi Fukumoto, et al. (2006) Laminin 5 is required for dental epithelium growth and polarity and the development of tooth bud and shape. Journal of Biological Chemistry 281, 5008-5016.
            • Ana Olivera, et al. (2006) IgE-dependent activation of sphingosine kinase 1 and 2 and secretion of sphingosine-1-phosphate requires Fyn kinase and contributes to mast cell responses. Journal of Biological Chemistry 281, 2515-2525.
            • Qian Yang, et al. (2006) TSC1/TSC2 and Rheb have different effects on TORC1 and TORC2 activity. Proceedings of the National Academy of Sciences (USA) 103, 6811-6816.
            • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
            • Huanyu Dou, et al. (2005) Neuroprotective mechanisms of lithium in murine human immunodeficiency virus-1 encephalitis. Journal of Neuroscience 25, 8375-8385.
            • Julie Lotharius, et al. (2005) Progressive degeneration of human mesencephalic neuron-derived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed lineage kinase pathway. Journal of Neuroscience 25, 6329-6342.
            • Meenalakshmi M. Mariappan, M.M., et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
            • Michael Tscharntke, et al. (2005) Ras-induced spreading and wound closure in human epidermal keratinocytes. The FASEB Journal in press,.
            • Junji Tsurutani, et al. (2005) Inhibition of the phosphatidylinositol 3-kinase/Akt/Mammalian target of rapamycin pathway but no the MEK/ERK Pathway attentuates laminin-meidated small cell lunch cancer cellular survival and resistance to Imatinib mesylate or chemolyte.. Cancer Research 65, 8423-8432.
            • Xinbo Liao, et al. (2003) Androgen stimulates matrix metalloproteinase-2 expression in human prostate cancer. Endocrinology 144, 1656-1663.
              		•