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PF059 Klenow Enzyme

Klenow Enzyme: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
PF059
  
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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The large fragment of DNA polymerase I. The Klenow fragment exhibits 5′ to 3′ DNA polymerase and 3′ to 5′ exonuclease activity, but lacks the 5′ to 3′ exonuclease activity of intact DNA polymerase I.
      Catalogue NumberPF059
      Brand Family Calbiochem®
      References
      ReferencesTelford, J.L. et al. 1979. Proc. Natl. Acad. Sci. USA 76, 2590.
      Wickens, M.P. 1978. J. Biol. Chem. 253, 2483.
      Sanger, F., et al. 1977. Proc. Natl. Acad. Sci. USA 74, 5463.
      Jaccobsen, H., et al. 1974. Eur. J. Biochem. 45, 623.
      Product Information
      Unit of DefinitionOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 min at 37°C.
      FormLiquid
      FormulationIn 50 mM potassium phosphate buffer, 0.25 mM dithiothreitol, 50% glycerol, pH 7.0.
      PreservativeNone
      Applications
      Application NotesDNA Labeling
      Application CommentsOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 minutes at 37°C.
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      PF059 0

      Documentation

      Klenow Enzyme Analysenzertifikate

      TitelChargennummer
      PF059

      Literatur

      Übersicht
      Telford, J.L. et al. 1979. Proc. Natl. Acad. Sci. USA 76, 2590.
      Wickens, M.P. 1978. J. Biol. Chem. 253, 2483.
      Sanger, F., et al. 1977. Proc. Natl. Acad. Sci. USA 74, 5463.
      Jaccobsen, H., et al. 1974. Eur. J. Biochem. 45, 623.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      ApplicationDNA Labeling
      DescriptionKlenow Enzyme is the large fragment of DNA polymerase I which can be produced by cleavage of intact DNA Polymerase I with subtilisin. The Klenow fragment exhibits 5’ → 3’ DNA polymerase and 3' → 5' exonuclease activity, but lacks the 5’ → 3’ exonuclease activity of intact DNA polymerase I. Klenow is used to fill in recessed 3’ termini as in end-labeling or in the synthesis of the second strand of cDNA from a single-stranded template. Klenow is also used in dideoxy sequencing reactions.
      BackgroundKlenow Enzyme is the large fragment of DNA polymerase I which can be produced by cleavage of intact DNA Polymerase I with subtilisin. The Klenow fragment exhibits 5’ → 3’ DNA polymerase and 3' → 5' exonuclease activity, but lacks the 5’ → 3’ exonuclease activity of intact DNA polymerase I. Klenow is used to fill in recessed 3’ termini as in end-labeling or in the synthesis of the second strand of cDNA from a single-stranded template. Klenow is also used in dideoxy sequencing reactions.
      FormLiquid
      FormulationIn 50 mM potassium phosphate buffer, 0.25 mM dithiothreitol, 50% glycerol, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 min at 37°C.
      SolubilityFollowing initial thaw, aliquot and freeze (-20°C).
      PreservativeNone
      CommentsOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 minutes at 37°C.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesTelford, J.L. et al. 1979. Proc. Natl. Acad. Sci. USA 76, 2590.
      Wickens, M.P. 1978. J. Biol. Chem. 253, 2483.
      Sanger, F., et al. 1977. Proc. Natl. Acad. Sci. USA 74, 5463.
      Jaccobsen, H., et al. 1974. Eur. J. Biochem. 45, 623.