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Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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SCR108
Sigma-AldrichHuman Mesenchymal Stem Cell Kit - Derived from Bone-Marrow
This Human Mesenchymal Stem Cell Kit contains 1 million viable cells & 500ml of optimized media.
More>>This Human Mesenchymal Stem Cell Kit contains 1 million viable cells & 500ml of optimized media. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
This Human Mesenchymal Stem Cell Kit contains 1 million viable cells & 500ml of optimized media.
Key Applications
Stem Cell Culture
Application Notes
Mesenchymal stem cells, also known as marrow stromal cells (1), are defined as a self-renewing population of adherent, bone-marrow-derived multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined in vitro assays, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissues (1, 2). Mesenchymal stem cells also provide support and maintenance for the other major stem cell population in the bone marrow, the hematopoietic stem cells (2). Mesenchymal stem cells (MSC’s) have historically been isolated based on the ability of these cells to form adherent cell layers in culture and the concomitant lack of adherence of other cells in the bone marrow stroma such as hematopoietic stem cells, adipocytes, and macrophage (1, 3). While this procedure results in enriched populations of mesenchymal stem cells, the resulting bone-marrow derived cell populations are, nonetheless, heterogeneous – comprised not only of mesenchymal stem cells, but also of committed lineage-restricted progenitors (1, 3). To aid researchers in the accurate identification and characterization of mesenchymal stem cells, Millipore provides cryopreserved Human Mesenchymal Stem Cells and optimized media in this kit. Millipore’s cryopreserved Human Mesenchymal Stem Cells are ready-to-use primary mesenchymal stem cells isolated from the iliac crest of normal human bone marrow (18-30 years old).
Biological Information
Source
Human bone marrow
Stem Cell Type
Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Millipore’s Human Mesenchymal Stem Cells are derived from human bone marrow and have been validated for high expression level of cell surface molecules that are present on mesenchymal stem cells: H-CAM (CD44), THY-1 (CD90) and STRO-1 and for their absence of hematopoietic cell surface markers, CD14 (present on leukocytes) and CD19 (present on B-lymphocytes). Millipore’s Human Mesenchymal Stem Cells are also negative for the endothelial marker, M-CAM (CD146). The cells have also been validated for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma. Each lot of Mesenchymal Stem Cell medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Human Mesenchymal Stem Cells (Part # SCC034) should be stored in liquid nitrogen. Mesenchymal Stem Cell Expansion Medium: (Catalog No. SCM015) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
Packaging Information
Material Size
1 kit containg >1 million viable cells and 500 mL of optimized media
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
SCR108
04053252005190
Documentation
Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow SDB
Self-organizing tissue-engineered constructs in collagen hydrogels. Robert G Gourdie,Tereance A Myers,Alex McFadden,Yin-Xiong Li,Jay D Potts Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
18
2011
A novel self-organizing behavior of cellularized gels composed of collagen type 1 that may have utility for tissue engineering is described. Depending on the starting geometry of the tissue culture well, toroidal rings of cells or hollow spheroids were prompted to form autonomously when cells were seeded onto the top of gels and the gels released from attachment to the culture well 12 to 24 h after seeding. Cells within toroids assumed distinct patterns of alignment not seen in control gels in which cells had been mixed in. In control gels, cells formed complex three-dimensional arrangements and assumed relatively higher levels of heterogeneity in expression of the fibronectin splice variant ED-A--a marker of epithelial mesenchymal transformation. The tissue-like constructs resulting from this novel self-organizing behavior may have uses in wound healing and regenerative medicine, as well as building blocks for the iterative assembly of synthetic biological structures.
