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03-182 HDAC2 (1-488) (His-tag) human recombinant

Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.

HDAC2 (1-488) (His-tag) human recombinant: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Key Applications: Enzyme Assays
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03-182
50 µg  
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      Replacement Information

      Key Spec Table

      Key ApplicationsSpecies
      Enzyme AssaysHuman
      Description
      Catalogue Number03-182
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHDAC2 (1-488) (His-tag) human recombinant
      OverviewHuman recombinant HDAC2 (1-488) (His-tag) produced in insect cells
      Background InformationHistone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It associates with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA. This process is highly conserved from yeast to human.
      References
      Product Information
      FormatPurified
      PresentationIn liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationHuman recombinant HDAC2 (1-488) (His-tag) produced in insect cells.
      Key Applications
      • Enzyme Assays
      Application NotesHDAC deacetylase assays
      Biological Information
      PurityPurity >50% by SDS-PAGE
      SourceResidues 1-488 of human HDAC2
      Specific Activity>5 U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity.
      Molecular Weight~55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC2 into separate tubes before storing at -80°C.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      03-182 04053252440731