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509227 G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 108321-42-2 - Calbiochem

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Synonyme: G418, G 418

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      Übersicht

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      108321-42-2C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Description
      OverviewThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
      Catalogue Number509227
      Brand Family Calbiochem®
      SynonymsG418, G 418
      References
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Product Information
      CAS number108321-42-2
      FormLiquid
      FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.
      Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Quality LevelMQ200
      Applications
      Biological Information
      Purity≥98% by TLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      S PhraseS: 36/37/39

      Wear suitable protective clothing, gloves and eye/face protection.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      509227 0

      Documentation

      G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 108321-42-2 - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Literatur

      Übersicht
      Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-December-2014 JSW
      SynonymsG418, G 418
      DescriptionThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
      FormLiquid
      FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.
      Intert gas (Yes/No) Packaged under inert gas
      CAS number108321-42-2
      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Purity≥98% by TLC
      Storage Protect from light
      Avoid freeze/thaw
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
      Toxicity Standard Handling
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.