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345810 G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

G418 also known as Geneticin® is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: NMD Inhibitor I, G418, G 418

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      Übersicht

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      108321-42-2C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Description
      OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
      Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).
      Catalogue Number345810
      Brand Family Calbiochem®
      SynonymsNMD Inhibitor I, G418, G 418
      References
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Product Information
      CAS number108321-42-2
      FormWhite powder
      Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityAssay: ≥730 µg/mg
      Purity≥98% by TLC
      Physicochemical Information
      ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSCB9378500
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      345810 0

      Documentation

      G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Analysenzertifikate

      TitelChargennummer
      345810

      Literatur

      Übersicht
      Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.

      Broschüre

      Titel
      Antibiotics Profiler
      Bulk Product Guide
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      Literaturstellen

      Titel
    • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
    • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
      		•
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-December-2011 JSW
      SynonymsNMD Inhibitor I, G418, G 418
      DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
      FormWhite powder
      Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
      CAS number108321-42-2
      RTECSCB9378500
      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Structure formulaStructure formula
      Purity≥98% by TLC
      ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
      Biological activityAssay: ≥730 µg/mg
      SolubilitySolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
      Toxicity Standard Handling
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Citation
    • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
    • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
      		•

      Verwandte Produkte & Anwendungen

      Alternative Products

      Bestellnummer Beschreibung  
      345812 G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Preis & Verfügbarkeit

      Kategorien

      Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest > Protein Synthesis Inhibitors
      Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents
      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents