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341583 FGF-2, Basic, Bovine

FGF-2, Basic, Bovine: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: nbbFGF, Fibroblast Growth Factor, Basic, Bovine

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      Übersicht

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells.
      Catalogue Number341583
      Brand Family Calbiochem®
      SynonymsnbbFGF, Fibroblast Growth Factor, Basic, Bovine
      References
      ReferencesOhmiya, M., et al. 2001. J. Neurosci. Res. 65, 228.
      Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
      Thomas, K. 1987. Methods Enzymol. 147, 120.
      Product Information
      CAS number106096-93-9
      FormLyophilized
      FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <0.1 ng/µg FGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSLK3641300
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      341583 0

      Documentation

      FGF-2, Basic, Bovine SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      FGF-2, Basic, Bovine Analysenzertifikate

      TitelChargennummer
      341583

      Literatur

      Übersicht
      Ohmiya, M., et al. 2001. J. Neurosci. Res. 65, 228.
      Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
      Thomas, K. 1987. Methods Enzymol. 147, 120.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-May-2008 RFH
      SynonymsnbbFGF, Fibroblast Growth Factor, Basic, Bovine
      DescriptionNative, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. A multifunctional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Known to activate phopholipase D in endothelial cells. Higher concentrations are found in the brain and pituitary gland. Purified from bovine brain by sequential chromatography.
      FormLyophilized
      FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.
      CAS number106096-93-9
      RTECSLK3641300
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: <0.1 ng/µg FGF
      Biological activityED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts
      SolubilityReconstitute to a concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 4072
      ReferencesOhmiya, M., et al. 2001. J. Neurosci. Res. 65, 228.
      Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
      Thomas, K. 1987. Methods Enzymol. 147, 120.

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      Kategorien

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors