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S-004-B Enzyme Free Cell Dissociation Solution Hank's Based (1X), liquid, 500ml

The Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.

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S-004-B
500 mL  
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      Replacement Information
      Description
      Catalogue NumberS-004-B
      Trade Name
      • Specialty Media
      DescriptionEnzyme Free Cell Dissociation Solution Hank's Based (1X), liquid, 500ml
      OverviewEnzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency.



      Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
      References
      Product Information
      PresentationProduct is supplied as a sterile liquid.
      Quality LevelMQ200
      Applications
      ApplicationThe Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsProduct can be stored at room temperature or at 4°C. Shelf life is 2 years.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      S-004-B 04053252661334