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324714 Endoproteinase Lys-C, Lysobacter enzymogenes

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      Übersicht

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide or ester bonds at the carboxylic side of lysine in peptides and proteins. Useful for sequence and structural analysis. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
      Catalogue Number324714
      Brand Family Calbiochem®
      References
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Product Information
      CAS number72561-05-8
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
      EC number3.4.21.50
      FormLyophilized
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥150 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      324714 0

      Documentation

      Endoproteinase Lys-C, Lysobacter enzymogenes Analysenzertifikate

      TitelChargennummer
      324714

      Literatur

      Übersicht
      Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide or ester bonds at the carboxylic side of lysine in peptides and proteins. Useful for sequence and structural analysis. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Has an optimal pH of 8.5-8.8.
      FormLyophilized
      Recommended reaction conditions
      Protocol Method 1: 1. Add the endoproteinase to the substrate at a concentration of 1:100 (protease:protein by weight) in 100 mM ammonium bicarbonate, pH 9.0. 2. Incubate for 1-2 h at 37°C. 3. Add a second aliquot or protease equal to the initial amount added and continue to incubate at 37°C for 11 h. Method 2: 1. Alternatively, mix the endoproteinase with the substrate at a concentration of 1:100 by weight in 100 mM ammonium bicarbonate, pH 8.5, containing SDS (1 mg/ml). 2. Incubate for 2 h at 37°C. 3. Add a second aliquot of protease equal to the initial amount added and incubate at 37°C. 4. Remove aliquots at timed intervals and monitor the progress of the digestion by electrophoresis.
      CAS number72561-05-8
      EC number3.4.21.50
      Specific activity≥150 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
      SolubilityH₂O
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Toxicity Harmful
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.