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371209 Dkk-2, Mouse, Recombinant

Dkk-2, Mouse, Recombinant, consists of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence.

Dkk-2, Mouse, Recombinant: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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371209
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Preis & Verfügbarkeit

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371209-10UG
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      Description
      OverviewRecombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
      Catalogue Number371209
      Brand Family Calbiochem®
      References
      ReferencesMorvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
      Mukhopadhyay, M. et al. 2006. Development 133, 2149.
      Niehrs, C. 2006. Oncogene 25, 7469.
      Li, X. et al. 2005. Nat. Genet. 37, 945.
      Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B. and C. Niehrs 2003. Gene 302, 179.
      Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
      Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
      Mao, B. et al. 2002. Nature 417, 664.
      Mao, B. et al. 2001. Nature 411, 321.
      Wu, W. et al. 2000. Current Biol. 10,1611.
      Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
      Krupnik, V.E. et al. 1999. Gene 238, 301.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityInhibition assay:
      Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

      Competetive Binding Assay:
      Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU per/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      371209-10UG 04055977213836

      Documentation

      Dkk-2, Mouse, Recombinant SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Dkk-2, Mouse, Recombinant Analysenzertifikate

      TitelChargennummer
      371209

      Literatur

      Übersicht
      Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
      Mukhopadhyay, M. et al. 2006. Development 133, 2149.
      Niehrs, C. 2006. Oncogene 25, 7469.
      Li, X. et al. 2005. Nat. Genet. 37, 945.
      Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B. and C. Niehrs 2003. Gene 302, 179.
      Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
      Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
      Mao, B. et al. 2002. Nature 417, 664.
      Mao, B. et al. 2001. Nature 411, 321.
      Wu, W. et al. 2000. Current Biol. 10,1611.
      Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
      Krupnik, V.E. et al. 1999. Gene 238, 301.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2011 RFH
      DescriptionRecombinant, mouse Dickkopf related protein 2 (Dkk-2) consisting of amino acids 26-259 and expressed in S. frugiperda 21 cells with a C-terminal His•Tag® sequence. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations.
      FormSolid
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU per/µg protein
      Biological activityInhibition assay:
      Inhibits WNT-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells in the presence of BMP-2.

      Competetive Binding Assay:
      Inhibits 50% binding of biotinylated rhDkk-1 (200 ng/ml) to immobilized rmKremen (4 µg/ml) at a concentration of 5-15 µg/ml.
      SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesMorvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
      Mukhopadhyay, M. et al. 2006. Development 133, 2149.
      Niehrs, C. 2006. Oncogene 25, 7469.
      Li, X. et al. 2005. Nat. Genet. 37, 945.
      Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
      Mao, B. and C. Niehrs 2003. Gene 302, 179.
      Brott, B. and S.Y. Sokol 2002. Mol. Cell. Biol. 22, 6100.
      Li, L. et al. 2002. J. Biol. Chem. 277, 5977.
      Mao, B. et al. 2002. Nature 417, 664.
      Mao, B. et al. 2001. Nature 411, 321.
      Wu, W. et al. 2000. Current Biol. 10,1611.
      Monaghan, A.P. et al. 1999. Mech. Dev. 87, 45.
      Krupnik, V.E. et al. 1999. Gene 238, 301.