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17-10530 ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set

Use ChIPAb+ MED1 ChIP Validated rabbit polyclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.

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Key Applications: Chromatin Immunoprecipitation (ChIP), Western Blotting Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors
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17-10530
25 assays  25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Key Spec Table

      Species ReactivityKey Applications
      H, MChIP, WB
      Description
      Catalogue Number17-10530
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ MED1 (CRSP1/TRAP220) set includes a MED1 antibody, a Normal Rabbit IgG, and positive control primers which amplify 85 bp of a human prostate-specific antigen (PSA) enhancer region. The MED1 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of MED1 associated chromatin.
      Alternate Names
      • CRSP1
      • TRAP220
      • MED1
      • TRIP-2
      • ARC205
      • Mediator of RNA polymerase II transcription subunit 1
      • Activator-recruited cofactor 205 kDa component
      • Mediator complex subunit 1
      • p53 regulatory protein RB18A
      • Peroxisome proliferator-activated receptor-binding protein
      • Thyroid receptor-interacting protein 2
      • PBP
      • PPAR-binding protein
      • Thyroid hormone receptor-associated protein complex 220 kDa component
      • Trap220
      • Thyroid receptor-interacting protein 2
      • TR-interacting protein 2
      • TRIP-2
      • Vitamin D receptor-interacting protein complex component DRIP205
      • p53 regulatory protein RB18A
      References
      Product Information
      FormatAffinity Purified
      Control
      • Controls
        Includes normal rabbit IgG and primers specific for a human prostate-specific antigen enhancer region.
      PresentationAnti-MED1 (Rabbit Polyclonal), Part No. CS207349. One vial containing 50 µg affinity purified rabbit polyclonal in 72 uL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7 mg/mL final). Store at -20°C.

      Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.

      ChIP Primers, PSA. Part No. CS207340. One vial containing 75 μL of 5 μM of each primer specific for human prostate-specific antigen enhancer region (chr19:51354179-51354263, hg19 build). Store at -20°C.
      FOR: GCC TGG ATC TGA GAG AGA TAT CAT C
      REV: ACA CCT TTT TTT TTC TGG ATT GTT G
      Quality LevelMQ100
      Applications
      ApplicationUse ChIPAb+ MED1 ChIP Validated rabbit polyclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340) as a positive locus, and ChIP Primers, β-globin (Part No. CS200591) as a negative locus.
      Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

      Chromatin Immunoprecipitation:
      Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No. CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
      Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

      Western Blotting Analysis:
      0.04 µg/mL of this antibody detected MED1 on 5, 15 and 50 µg of HeLa whole cell lysate.
      Biological Information
      ImmunogenRecombinant protein corresponding to human MED1 (a.a. 1525-1581).
      EpitopeHuman MED1 (a.a. 1525-1581).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Wide species reactivity expected based upon conservation of immunogen sequence.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARC2052
      • CRSP1
      • CRSP200
      • DRIP205
      • DRIP230
      • PBP
      • PPARBP
      • PPARGBP
      • RB18A
      • TRAP220
      • TRIP
      UniProt Number
      Molecular Weight~168 kDa based on sequence. Higher mw up to 220 kDa observed on western blots.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from hormone starved (72h), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
      Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      17-10530 04053252947919

      Documentation

      ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set Analysenzertifikate

      TitelChargennummer
      ChIPAb+ MED1 - Q2370588 Q2370588
      ChIPAb+ MED1 - 3176293 3176293
      ChIPAb+ MED1 - 3282684 3282684
      ChIPAb+ MED1 - 3386083 3386083
      ChIPAb+ MED1 - 3386606 3386606
      ChIPAb+ MED1 - 3503505 3503505
      ChIPAb+ MED1 - 3590174 3590174
      ChIPAb+ MED1 - 3789473 3789473
      ChIPAb+ MED1 - 3865030 3865030
      ChIPAb+ MED1 - 3931645 3931645
      Show more

      Broschüre

      Titel
      Advance your Epigenetics Research