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QIA20 Cell Death Detection (Nuclear Matrix Protein) ELISA Kit

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Detection Methods: Colorimetric
QIA20
  
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      Übersicht

      Key Spec Table

      Detection Methods
      Colorimetric
      Description
      Overview

      This product has been discontinued.





      Designed for the quantitative detection of NMP41/7. The specific detection of soluble human NMPs afforded by the Cell Death Detection (Nuclear Matrix Protein) ELISA allows quantitation of cell death.
      Catalogue NumberQIA20
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Precision pipettors with disposable tips
      • Wash bottle or multichannel dispenser for washing
      • Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450/595 nm.
      References
      ReferencesMiller, T.E., et al. 1994. In Current Communications in Cellular and Molecular Biology: Apoptosis II. 8, 357.
      Arguello, F., et al. 1993. Blood 82, 999a.
      Miller, T.E., et al. 1993. Biotechniques 15, 1042.
      Partin, A.W., et al. 1993. Cancer Res. 53, 744.
      Compton, D.A., et al. 1992. J. Cell Biol. 116, 1395.
      Miller, T.E., et al. 1992. Cancer Res. 52, 422.
      Yang, C.H., et al. 1992. J. Cell Biol. 116, 1303.
      Nakayasu, H. and R. Berezney. 1989. J. Cell Biol. 108, 1.
      Fey, E.G. and S. Penman. 1988. Proc. Natl. Acad. Sci. USA 85, 121.
      Zeitlin, S., et al. 1987. Mol. Cell Biol. 7, 111.
      Kumara-Siri, M.H. 1986. Biol. Chem. 261, 2844.
      Berrios, M., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4142.
      Pardoll, D.M., et al. 1980. Cell 19, 527.
      Berezney, R. and D.S. Coffey. 1974. Biochem. Biophys. Res. Commun. 60, 1410.
      Product Information
      Unit of DefinitionOne unit (U) is defined as the amount of NMP 41/7 released by 850 MCF7 cells maintained at a density of 1 - 5 x 10⁵ cells/ml in serum-free medium for 7 days.
      Detection methodColorimetric
      DeclarationUse of this product is covered by U.S. Patents 5,783,403, 5,882,876, 5,989,826, 6,162,607, 6,410,247, and 6,740,494. Foreign patents also cover the use of this product in numerous jurisdictions.
      Form96 Tests
      Format96-well plate
      Kit containsCoated 96-Well Removable Strip Plate, Lyophilized NMP Standard, NMP Detector Antibody, Anti-FITC-POD, Substrate, ELISA Conjugant Diluent, 20X Plate Wash Concentrate, Stop Solution, Plate Sealer, and a user protocol.
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Biological Information
      Assay range15-1000 U/ml
      Assay time4 h
      Sample TypeTissue culture supernatant
      Physicochemical Information
      Sensitivity10 U/ml
      Product Usage Statements
      Intended useThe Calbiochem® Cell Death Detection (Nuclear Matrix Protein) ELISA Kit is used for quantitative determination of nuclear matrix protein 41/7, which is released from dead and dying cells. Nuclear matrix protein 41/7 appears to be the same as that designated as NuMA (nuclear mitotic apparatus protein) by some researchers.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Storage ConditionsUpon arrival all components should be stored at -20°C. Coated assay plates should be stored in the original foil bag sealed by the zip lock and containing a desiccant pack. All of the reagents included in the Cell Death Detection (Nuclear Matrix Protein) ELISA have been tested for stability.
      Do not freeze Yes
      Supplemental Information
      Kit containsCoated 96-Well Removable Strip Plate, Lyophilized NMP Standard, NMP Detector Antibody, Anti-FITC-POD, Substrate, ELISA Conjugant Diluent, 20X Plate Wash Concentrate, Stop Solution, Plate Sealer, and a user protocol.
      Global Trade Item Number
      Bestellnummer GTIN
      QIA20 0