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L6715-BC Biocoll 1.077

L6715-BC
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L6715
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      		 500 ml
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      Description
      OverviewBiocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. Densities of up to 1.1 g/ml can be adjusted using this hydrophilic polymer. For optimal pH and osmolality, adjusting Biocoll with an acid, preferably amidotrizoeic acid(ATA), and sodium hydroxide is required. Biocoll with densities of 1.077 g/ml (Cat. No. L6113/5) and 1.090 g/ml (Cat. No. L6125) are already adjusted accordingly.

      Specifications:

      Sterility: Pass
      Functional Test: Pass
      pH: 7.0-7.5
      Osmolality: 0.290-0.320 Osm/kg H20
      Endotoxin: < 1.0 EU/ml
      Density: 1.076-1.078 g/ml
      Color: Colorless
      Appearance: Clear
      Separating solutions

      Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.


      Recommendations:

      The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. But you can also adjust a required density yourself diluting Easycoll correspondingly.
      Table 36: Instruction how to dilute the stock solution of Biocoll (density 1.090 g/ml; cat. no. L 6125) with PBS (cat. no. L 1825/0)
      All amounts given for a temperature of +20°C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions will influence agglomeration and increase formation of cell clusters.

      Separating lymphocytes using Biocoll:

      1. Biocoll separating solution (D = 1.077 at +20°C) is given to 15 or 25 ml centrifugal tubes in volumes of 7 ml and 10 ml, resp.
      2. Equal parts of heparinized whole blood (50 U/ml heparin, stabilizer-free) and culture medium are mixed and carefully applied over Biocoll separating solution.
      3. Centrifuge 1200xg for 20 min.
      4. Take layer of enriched (70 - 100 %) lymphocytes (between plasma and Biocoll) with a Pasteur′s pipette and wash twice in culture medium:
      • for 10 min at 300xg
      • for 10 min at 200xg
      5. Cell count as usual.
      Catalogue NumberL6715-BC
      Brand Family Calbiochem®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      L6715 04027269841362

      Documentation