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OP07L Anti-v-Src (Ab-1) Mouse mAb (327)

Anti-v-Src (Ab-1) Mouse mAb (327): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
      Catalogue NumberOP07L
      Brand Family Calbiochem®
      References
      ReferencesCourtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlNIH/3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Frozen Sections
      Not Immunofluorescence
      Not Paraffin Sections
      Application NotesFrozen Sections (not recommended)
      Immunoblotting (2.5 µg/ml, see application references)
      Immunofluorescence (not recommended)
      Immunoprecipitation (1 µg/sample or use Cat. No. OP07A; see application references)
      Paraffin Sections (not recommended)
      Application Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src kinase(see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified v-Src
      Epitopewithin the SH3 domain of v-Src and c-Src
      Clone327
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, refrigerate (4°C) with 0.1% azide for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid repeated freeze/thaw cycles of solutions. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      OP07L 0

      Documentation

      Anti-v-Src (Ab-1) Mouse mAb (327) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-v-Src (Ab-1) Mouse mAb (327) Analysenzertifikate

      TitelChargennummer
      OP07L

      Literatur

      Übersicht
      Courtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.

      Literaturstellen

      Titel
    • Meenalakshmi M. Mariappan, et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
    • Justin M. Summy, et al. (2005) AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes. Molecular Cancer Therapeutics 4, 1900-1911.
    • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
      		•
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationFrozen Sections (not recommended)
      Immunoblotting (2.5 µg/ml, see application references)
      Immunofluorescence (not recommended)
      Immunoprecipitation (1 µg/sample or use Cat. No. OP07A; see application references)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
      BackgroundThe major translational product of the avian sarcoma virus has been designated pp60src. Avian sarcoma virus pp60src possesses a characteristic protein kinase activity with specificity for tyrosine residues. The mammalian cellular homologue of pp60src, designated pp60c-src, exhibits a similar protein tyrosine kinase activity which can be activated by interaction with polyoma virus-encoded middle T antigen. Immunoprecipitates of middle T antigen also contain phosphatidylinositol kinase activity coinciding with the presence of a 81 kDa (p81) protein. The p81 is phosphorylated by activated pp60c-src and is thought to be the phosphatidylinositol kinase.
      HostMouse
      Immunogenpurified v-Src
      Epitopewithin the SH3 domain of v-Src and c-Src
      Clone327
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlNIH/3T3 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src kinase(see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, refrigerate (4°C) with 0.1% azide for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid repeated freeze/thaw cycles of solutions. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h.
      Toxicity Standard Handling
      ReferencesCourtneidge, S.A. and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Citation
    • Meenalakshmi M. Mariappan, et al. (2005) Phospholipase Cγ-Erk Axis in Vascular Endothelial Growth Factor-Induced Eukaryotic Initiation Factor 4E Phosphorylation and Protein Synthesis in Renal Epithelial Cells. Journal of Biological Chemistry 280, 28402-28411.
    • Justin M. Summy, et al. (2005) AP23846, a novel and highly potent Src family kinase inhibitor, reduces vascular endothelial growth factor and interleukin-8 expression in human solid tumor cell lines and abrogates downstream angiogenic processes. Molecular Cancer Therapeutics 4, 1900-1911.
    • Jose G. Trevino, et al. (2005) Expression and activity of Src regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis. Cancer Research 65, 7214-7222.
      		•
      Application referencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies