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07-449-AF488 Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate

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07-449-AF488
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue Number07-449-AF488
      DescriptionAnti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      • trimethyl-Histone (Lys27), Alexa Fluor® 488 Conjugate
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. 07-449) is shown to be suitable also for immunohistochemistry, immunocytochemistry, immunoprecipitation, multiplex assay, Western blotting, chromatin immunoprecipitation (ChIP), and ChIP-seq applications.
      Biological Information
      ImmunogenKLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(Kme3)SAP… in which Kme3 corresponds to trimethyl-lysine at residue 27 of human histone H3.
      EpitopeH3K27me3
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityH3K27me3 specificity of the unconjugated antibody (Cat. No. 07-449) has been confirmed using the Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Broad species cross-reactivity expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Modifications
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      07-449-AF488 04055977298246

      Documentation

      Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor® 488 Conjugate Analysenzertifikate

      TitelChargennummer
      Anti-trimethyl-Histone H3 (Lys27), -Q2623736 Q2623736
      Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate 2911833
      Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 3487544 3487544
      Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 3677706 3677706
      Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 3864997 3864997
      Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 4045886 4045886
      Anti-trimethyl-Histone H3 (Lys27), Alexa Fluor® 488 Conjugate - 4095797 4095797