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MABS1262 Anti-phospho-ZIP7 (Ser275/276) Antibody, clone 3-3-3

Detect ZIP7 using this mouse monoclonal Anti-phospho-ZIP7 (Ser275/276) Antibody, clone 3-3-3, Cat. No. MABS1262, validated for use in Immunocytochemistry and Western Blotting.

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Research Category: Signaling Gene Symbol: SLC39A7, HKE4, RING5 UniProt Number: Q92504
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MABS1262
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1262
      DescriptionAnti-phospho-ZIP7 (Ser275/276) Antibody, clone 3-3-3
      Alternate Names
      • Zinc transporter SLC39A7, S256/S257-phosphorylated
      • Histidine-rich membrane protein Ke4, S256/S257-phosphorylated
      • Really interesting new gene 5 protein, S256/S257-phosphorylated
      • Solute carrier family 39 member 7, S256/S257-phosphorylated
      • ZIP7, S256/S257-phosphorylated
      • Zrt-, Irt-like protein 7, S256/S257-phosphorylated
      Background InformationZinc transporter SLC39A7 (UniProt Q92504; also known as Histidine-rich membrane protein Ke4, Really interesting new gene 5 protein, Solute carrier family 39 member 7, ZIP7, Zrt-, Irt-like protein 7) is encoded by the SLC39A7 (also known as HKE4, RING5) gene (Gene ID 7922) in human. Zinc transporters mediate the transport of zinc ions into intracellular compartments as well as the export of excess zinc out of cells. Disruption of zinc homeostasis is responsible for many chronic neurodegenerative diseases and acute neural injuries. Two classes of multi-pass transmembrane Zn transporters are known, including 10 ZnTs and 14 ZIPs, that exhibit opposing functions in mediating zinc homeostasis. ZIP7 serves as a gatekeeper of zinc release from intracellular stores that transports zinc from the endoplasmic reticulum/Golgi apparatus to the cytosol. This transport is stimulated by certain growth calcium, and exogenous zinc. ZIP7 is activated following its phosphorylation at Ser275 and Ser276 by casein kinase 2 (CK2). ZIP7-mediated zinc release from intracellular stores is shown to drive major pathways, such as MAPK, mTOR and PI3K-AKT that are involved in cell survival and proliferation and are activated in cancer cells. Elevated ZIP7 levels are reported in most metastatic melanoma cell lines and in some brain and colon cancer cell lines. Under hyperglycemic conditions, as in diabetes, alterations in ZIP7 phosphorylation have been reported along with opposing changes in the expression of ZIP7 and ZnT7, which induce marked redistribution of cellular free zinc with an increase in cytosolic free zinc and a reduction in sarco(endo)plasmic reticulum. These changes may contribute to cardiac dysfunction commonly observed in diabetes. (Ref.: Nimmanon, T., et al. (2017). Metallomics. 9(5):471-481; Tuncay, E., et al. (2017). Diabetes. 66(5):1346-1358).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect ZIP7 using this mouse monoclonal Anti-phospho-ZIP7 (Ser275/276) Antibody, clone 3-3-3, Cat. No. MABS1262, validated for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: Clone 3-3-3 hybridoma culture supernatant (1:100 dilution) immunostained 3.7% paraformaldehyde-fixed, 0.4% saponin-permeabilized MCF-7 cells transfected with wild-type and S256D/S257D, but not S256A/S257A, ZIP7 construct (Courtesy of Kathryn Taylor, Ph.D., Cardiff University, United Kingdom).

      Western Blotting Analysis: Clone 3-3-3 hybridoma culture supernatant (1:1,000 dilution) detected a ~48 kDa phosphorylated target band in lysate from wild-type ZIP7-transfected MCF-7 cells (Courtesy of Kathryn Taylor, Ph.D., Cardiff University, United Kingdom).
      Biological Information
      ImmunogenKLH-conjugated phospho-peptide corresponding to the target region sequence with phosphorylated S256 and S257 residues.
      Clone3-3-3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3-3-3 reconizes S256/S257 dual phosphorylated wild-type ZIP7, as well as S256D/S257D, but not S256A/S257A, ZIP7 mutant.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Canine, Non-Human Primate, Porcine, Sheep based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC39A7
      • HKE4
      • RING5
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~48 kDa observed. 50.12 kDa (human ZIP7) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZIP7 S256/S257 phosphorylation in 20 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1262 04054839069581