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AB1956-I-200UL Anti-phospho-VE-cadherin (Tyr731)

Anti-phospho-VE-cadherin (Tyr731), Cat. No. AB1965-I, is a rabbit polyclonal antibody that detects Cadherin-5 phosphorylated on Tyrosine 731 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-phospho-VE-cadherin (Tyr731): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: CDH5 UniProt Number: P33151
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AB1956-I-200UL
200 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1956-I-200UL
      DescriptionAnti-phospho-VE-cadherin (Tyr731)
      Alternate Names
      • Cadherin-5
      • 7B4 antigen
      • Vascular endothelial cadherin
      • CD144
      Background InformationCadherin-5 (UniProt: P33151; also known as 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144) is encoded by the CDH5 gene (Gene ID: 1003) in human. Cadherins are calcium-dependent cell adhesion proteins, which preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 5 is a single-pass type I membrane protein that is found at cell-cell boundaries and may also be located at cell-matrix boundaries. It is well expressed in brain and endothelial tissue. It plays an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Cadherin 5 acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Cadherin 5 is synthesized as a preproprotein with a signal peptide (aa 1-25) and propeptide (aa 26-47) that are proteolytically cleaved to produce the mature glycoprotein. It has an extracellular domain (aa 48-599), a short transmembrane domain (aa 600-620), and a cytoplasmic domain (aa 621-784) and contains five cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, providing a strong curvature to the full-length ectodomains. Two isoforms of Cadherin 5 have been described that are produced by alternative splicing. Cadherin 5 can be phosphorylated on tyrosine residues by KDR/VRGFR-2. Phosphorylation at Tyr731 leads to the separation of beta-catenin from the cytoplasmic tail Cadherin 5. This phosphorylation event reported to be sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-VE-cadherin (Tyr731), Cat. No. AB1965-I, is a rabbit polyclonal antibody that detects Cadherin-5 phosphorylated on Tyrosine 731 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:1,500 dilution from a representative lot was used with HUVEC stimulated with VEGF for peptide block analysis.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphorylated tyrosine 731 from human Cadherin-5.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human and murine Cadherin-5 phosphorylated on Tyrosine 731.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDH5
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~100 kDa observed; 87.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HUVEC treated with VEGF.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-VE-cadherin (Tyr731) in lysate from HUVEC treated with VEGF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB1956-I-200UL 04054839482281