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ABF1207-AF488 Anti-phospho-Syk (Tyr323) Antibody, Alexa Fluor® 488 Conjugate

Detect Syk phosphorylated on tyrosine 323 using this rabbit polyclonal Anti-phospho-Syk (Tyr323), Alexa Fluor® 488 Conjugate, Cat. No. ABF1207-AF488, validated for use in Immunocytochemistry.

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Research Category: Signaling Gene Symbol: SYK UniProt Number: P43405
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ABF1207-AF488
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue NumberABF1207-AF488
      DescriptionAnti-phospho-Syk (Tyr323) Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Tyrosine-protein kinase Syk
      • p72-Syk
      • p72Syk
      • Spleen tyrosine kinase
      Background InformationTyrosine-protein kinase Syk (EC 2.7.10.2; UniProt P43405; also known as p72-Syk, p72Syk, Spleen tyrosine kinase) is encoded by the SYK gene (Gene ID 6850) in human. Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase highly expressed by haematopoietic cells. Syk plays a crucial role not only in adaptive immune receptor signalling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk adopts an autoinhibited conformation in its inactive state and is activated upon binding immunoreceptor tyrosine-based activation motifs (ITAMs) and phosphorylation of tyrosine residues in the two linker regions (a.a. 108-160 and 260-370) between SH1 (a.a. 15-107) and SH2 (a.a. 168-259), and between SH2 and the kinase domain (a.a. 371-631). Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. In additon, Syk can phosphorylate ITAMs and autophosphorylate its own link region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk also mediates signalling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR gamma-chain (FcRgamma).
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit polyclonal antibody Alexa Fluor® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Syk phosphorylated on tyrosine 323 using this rabbit polyclonal Anti-phospho-Syk (Tyr323), Alexa Fluor® 488 Conjugate, Cat. No. ABF1207-AF488, validated for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. ABF1207) is also available for Western blotting application.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr323 of human Syk.
      Epitopedomain of:
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityTarget phosphotyrosine residue coresponds to pTyr323 of human Syk spliced isoform 1 and pTyr300 of human Syk spliced isoform 2. Sequence surrounding the target tyrosine is not well conserved in mouse and rat Syk.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SYK
      Modifications
      • Phosphorylation
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~72/69 kDa observed. 72.07/69.51 kDa (human Syk isoform 1/2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Jurkat cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Syk Tyr323 phosphorylation induction in hydrogen peroxide-treated Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABF1207-AF488 04054839132674