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Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay.
More>>Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
There are two kinds of IGF receptors based on their relative affinities for IGF-1 versus IGF-2. The IGF-1 receptor (IGF-1R) prefers IGF-1 over IGF-2 and weakly binds insulin. IGF-1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF-1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF-1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by mediating signaling pathways that enhance cell survival.
References
Product Information
Format
Affinity Purified
Control
IGF1 untreated and treated HEK293 cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay.
Key Applications
Western Blotting
Cell Function Assay
Application Notes
Western Blot Peptide Inhibition Analysis: A representative lot detected IGF-1R in IGF1 treated HEK293 cell lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166.
Epitope
Phosphorylated Tyr1161/Tyr1165/Tyr1166.
Host
Rabbit
Specificity
This antibody recognizes IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166. Please note that some sources list the phsophorylation sites as Tyr1131/Tyr1135/Tyr1136. The difference in the amino acid sequence is due to a 30 amino acid signal chain that is removed due to protein processing.
Species Reactivity
Human
Rat
Mouse
Pig
Xenopus
Chicken
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Rat, Mouse, Porcine, Xenopus, Chicken, and Zebra Fish based on 100% sequence homology.
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
FUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. Hybrid receptors composed of IGF1R and INSR isoform Long are activated with high affinity by IGF1 and with low affinity by IGF2, but are not significantly activated by insulin. On the other hand, hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. Additionally, hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, where both receptor types bind IGF1 and have a low affinity for insulin.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
COFACTOR: Manganese.
ENZYME REGULATION: Autophosphorylation activates the kinase activity.
SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with GNB2L1/RACK1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
Molecular Weight
~95 kDa observed. IGF-1R can be cleaved into an Insulin-like growth factor 1 receptor alpha (aa 31-736) and beta (aa 741-1367) chain. Both chains are known to contain multiple potential glycosylation sites. Distinct beta-subunits are present in hybrid insulin-like-growth-factor-1 receptors in the central nervous system. (Moss, A.M., et al. (1993). Biochem J. 294( Pt 3):685-692.)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in IGF1treated HEK293 cell lysate.
Western Blot Analysis: A 1:200 dilution of this antibody detected IGF-1R in IGF1 treated HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
