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MABE943 Anti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10

Anti-phospho-Histone H3.3 Antibody, clone 1A8G10 is an antibody against phospho-Histone H3.3 for use in Western Blotting, ELISA.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: H3F3A, H3.3A, H3F3, H3F3B, H3.3B
 UniProt Number: P84243
MABE943
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE943
      DescriptionAnti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10
      Alternate Names
      • Histone H3.3 (Ser31)
      • Histone H3.3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Cat. No. MABE943, Anti-phospho-Histone H3.3 (Ser31), clone 1A8G10 detects Histone H3.3 phosphorylated on Ser31. Sequence surrounding the target H3.3 phosphorylattion site is conserved in H3.3 of human, murine, and rat species (UniProt P84243, P84244, and P84245, respectively), but not in H3.1 or H3.2.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-phospho-Histone H3.3 Antibody, clone 1A8G10 is an antibody against phospho-Histone H3.3 for use in Western Blotting, ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected phospho-Histone H3.3 (Ser31) in nocodazole treated HeLa cell lysate (Yoshimi, T., et al. (2013). Monoclon. Antib. Immunodiagn. Immunother. 32(2):119-124).
      ELISA Analysis: A representative lot detected phospho-Histone H3.3 (Ser31) peptides (Yoshimi, T., et al. (2013). Mono. Anti. In Immunodiagnosis and Immunotherapy. 32(2):119-124).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho-Histone H3.3 (Ser31).
      EpitopeSurrounding H3.3 pSer31
      Clone1A8G10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      • H3F3B
      • H3.3B
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in nocodazole treated Hela cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho-Histone H3.3 (Ser31) in 10 µg of nocodazole treated Hela cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE943 04055977164145

      Documentation

      Anti-phospho-Histone H3.3 (Ser31) Antibody, clone 1A8G10 Analysenzertifikate

      TitelChargennummer
      Anti-phospho-Histone H3.3 (Ser31), -Q2564185 Q2564185
      Anti-phospho-Histone H3.3 (Ser31), clone 1A8G10 - 3328851 3328851

      Literatur

      ÜbersichtPub Med ID
      A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
      Yoshimi, T; Ohkawa, Y; Azuma, M; Tachibana, T
      Monoclonal antibodies in immunodiagnosis and immunotherapy  32  119-24  2013

      Abstract anzeigen       		23607347 23607347

      Broschüre

      Titel
      NPI Flyer- Epigenetics and Nuclear Function Feature

      Technische Informationen

      Titel
      White Paper: Further considerations of antibody validation and usage.