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This Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5 is validated for use in western blotting, dot blot, ELISA & ICC for the detection of phospho Histone H3 (Thr11).
More>>This Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5 is validated for use in western blotting, dot blot, ELISA & ICC for the detection of phospho Histone H3 (Thr11). Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5 is validated for use in western blotting, dot blot, ELISA & ICC for the detection of phospho Histone H3 (Thr11).
Key Applications
Western Blotting
Dot Blot
Enzyme Immunoassay (ELISA)
Immunocytochemistry
Application Notes
Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa cell lysate.
Dot Blot (Specificity) Analysis: 0.2 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in an Absurance® Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance® Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells undergoing interphase and metaphase (Prof. Taro Tachibana, Cell Engineering Corporation.).
ELISA Analysis: A representative lot detected phospho Histone H3 (Thr11) in a panel of Histone H3 peptides (Prof. Taro Tachibana, Cell Engineering Corporation.).
Western Blotting Analysis: A representative lot detected phospho Histone H3 (Thr11) in HCT116 cell lysates, which were incubated for a period of time with either nocodazole or UV. The same lot detected phospho Histone H3 (Thr11) in HeLa cell lysates, which were incubated for a period of time with nocodazole. Additionally, the same lot detected phospho Histone H3 (Thr11) in tsFT210 whole cell extracts after released G2/M syncronization (Prof. Taro Tachibana, Cell Engineering Corporation.).
Western Blotting Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in nocodazole treated HeLa extract (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.)
ELISA Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in a panel of various phosphorylated H3 peptides (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in mitotic DM4 cells (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr11.
~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in nocodazole treated and untreated HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
A panel of specific monoclonal antibodies directed against various phosphorylated histones H3. Yoshimi, T; Ohkawa, Y; Azuma, M; Tachibana, T Monoclonal antibodies in immunodiagnosis and immunotherapy
32
119-24
2013
Modification of histone plays a critical role in the epigenetic regulation of gene expression. However, unlike the widely studied roles of histone methylation or acetylation of histone H3, relatively little is known about the molecular mechanisms involved in translating histone phosphorylation into a specific outcome. The present study reports on the development of antibodies (MAbs) directed against phosphorylated histone H3 (S10, T11, S28, S31, and T32), produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat or mouse. The MAbs produced specifically recognize different sites of phosphorylation on histone H3. All of these MAbs are suitable for immunoblotting and immunofluorescence analysis. We believe that these antibodies should significantly facilitate our efforts to investigate epigenetic regulation.
